製品の概要

  • 製品名
    Anti-Melanoma gp100 antibody [HMB45]
    Melanoma gp100 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HMB45] to Melanoma gp100
  • 由来種
    Mouse
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Rat, Dog
  • 免疫原

    Tissue, cells or virus corresponding to Human Melanoma. BALB/C mice were injected with extract of pigmented malanoma metastases from lymph nodes.
    Database link: P40967

  • ポジティブ・コントロール
    • IHC-P: Human melanoma and testis tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 0.5 - 1 µg/ml.

Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

ICC/IF Use a concentration of 0.5 - 1 µg/ml. PubMed: 19841138

ターゲット情報

  • 機能
    Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
  • 組織特異性
    Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
  • 配列類似性
    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • ドメイン
    The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
    The lumenal domain is necessary for correct processing and trafficking to melanosomes.
  • 翻訳後修飾
    A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
    N-glycosylated. O-glycosylated; contains sialic acid.
  • 細胞内局在
    Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 kDa melanocyte specific secreted glycoprotein antibody
    • 95 kDa melanocyte-specific secreted glycoprotein antibody
    • D12S53E antibody
    • gp100 antibody
    • M-beta antibody
    • ME20 antibody
    • ME20 M/ME20 S antibody
    • ME20-M antibody
    • ME20-S antibody
    • ME20M antibody
    • ME20M/ME20S antibody
    • ME20S antibody
    • Melanocyte lineage specific antigen GP100 antibody
    • Melanocyte protein mel 17 antibody
    • Melanocyte protein Pmel 17 antibody
    • Melanocyte protein Pmel 17 precursor antibody
    • Melanocytes lineage-specific antigen GP100 antibody
    • Melanoma associated ME20 antigen antibody
    • Melanoma gp100 antibody
    • Melanoma-associated ME20 antigen antibody
    • Melanosomal matrix protein 17 antibody
    • Melanosomal matrix protein17 antibody
    • P1 antibody
    • p100 antibody
    • p26 antibody
    • PMEL 17 antibody
    • PMEL antibody
    • PMEL_HUMAN antibody
    • PMEL17 antibody
    • Premelanosome protein antibody
    • Secreted melanoma-associated ME20 antigen antibody
    • SI antibody
    • SIL antibody
    • SILV antibody
    • Silver (mouse homolog) like antibody
    • Silver homolog antibody
    • Silver locus protein homolog antibody
    • Silver, mouse, homolog of antibody
    see all

画像

  • Formalin-fixed, paraffin-embedded human melanoma tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis. 

  • ab787 - immunohistochemistry

    Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

  • Formalin-fixed, paraffin-embedded human testis tissue stained with ab787 at 1 μg/ml in immunohistochemical analysis. 

  • ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

    See Abreview

  • Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

参考文献

This product has been referenced in:
  • Bergam P  et al. ABCB6 Resides in Melanosomes and Regulates Early Steps of Melanogenesis Required for PMEL Amyloid Matrix Formation. J Mol Biol N/A:N/A (2018). Read more (PubMed: 29940187) »
  • Behera R  et al. Inhibition of Age-Related Therapy Resistance in Melanoma by Rosiglitazone-Mediated Induction of Klotho. Clin Cancer Res 23:3181-3190 (2017). Read more (PubMed: 28232477) »
See all 15 Publications for this product

レビューと Q&A

1-5 of 5 Abreviews or Q&A

Answer



We don't have strict guidelines for length of storage for antibodies. In general, for antibody products stored at 4 degrees C, we would expect them to be stable for at least one year from date of purchase.

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Question
Answer

Merci de nous avoir contactés; ma collègue m'a fait part de votre appel.

Je suis heureux de savoir que l'anticorps a finalement fonctionné en cytométrie en flux. J'ai compris que vous deviez utiliser beaucoup d'anticorps pour obtenir des résultats satisfaisants sur 300.000 cellules. J'ai donc mis en place l'envoi d'une unité gratuite d'ab787 pour que vous puissiez terminer vos travaux avec cet anticorps.

Le numéro de la commande est ****.

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Thank you for contacting us.

Unfortunately, the blocking peptide for ab787 antibody is not available for purchase. We have ˜25 anti melanoma antibodies available but we do not have antigen available for any one of these however if you could select few as per your requirement then I will check with our collaborator laboratory if they can supply the peptide as special request.

We do have antibody / antigen pair for S100 protein. Please check the catalogue number ab14688 and ab30380.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma cell line WM3248 xenopraph)
Specification
Melanoma cell line WM3248 xenopraph
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Dakocytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Hongwei Shao

Verified customer

投稿 Feb 25 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (transfected HeLa)
Loading amount
20 µg
Specification
transfected HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Guillaume Van Niel

Verified customer

投稿 Jul 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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