Anti-Melanoma gp100 抗体 [EPR4864] (ab137062)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4864] to Melanoma gp100
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Melanoma gp100 antibody [EPR4864]
Melanoma gp100 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4864] to Melanoma gp100 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human melanoma tissue lysate; Human melanoma tissue ;Placenta tissue; Normal skin tissue;Normal kidney tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4864 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137062の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 100, 26 kDa (predicted molecular weight: 70 kDa).
For unpurified, use 1/1000 - 1/2000. |
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IHC-P |
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified, use 1/50 - 1/100. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 100, 26 kDa (predicted molecular weight: 70 kDa). For unpurified, use 1/1000 - 1/2000. |
IHC-P
1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/50 - 1/100. |
ターゲット情報
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機能
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. -
組織特異性
Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. -
配列類似性
Belongs to the PMEL/NMB family.
Contains 1 PKD domain. -
ドメイン
The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
The lumenal domain is necessary for correct processing and trafficking to melanosomes. -
翻訳後修飾
A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
N-glycosylated. O-glycosylated; contains sialic acid. -
細胞内局在
Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 6490 Human
- Omim: 155550 Human
- SwissProt: P40967 Human
- Unigene: 95972 Human
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別名
- 95 kDa melanocyte specific secreted glycoprotein antibody
- 95 kDa melanocyte-specific secreted glycoprotein antibody
- D12S53E antibody
see all
画像
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Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/10000 dilution (purified) + Human melanoma tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human melanoma with purified ab137062 at a working dilution of 1 in 400. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Anti-Melanoma gp100 antibody [EPR4864] (ab137062) at 1/1000 dilution (unpurified) + Human melanoma tissue lysate at 10 µg
Secondary
HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 70 kDa -
Immunohistochemical staining of paraffin embedded human melanoma with unpurified ab137062 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Immunohistochemical analysis using unpurified ab137062 showing negative staining in placenta tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis using unpurifed ab137062 showing negative staining in normal skin tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis using unpurified ab137062 showing negative staining in normal stomach tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab137062 は 10 報の論文で使用されています。
- Yamasaki S et al. Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture. Regen Ther 19:24-34 (2022). PubMed: 35059477
- Senabouth A et al. Transcriptomic and proteomic retinal pigment epithelium signatures of age-related macular degeneration. Nat Commun 13:4233 (2022). PubMed: 35882847
- Mitchell SM et al. Identification of critical amino acid residues in the regulatory N-terminal domain of PMEL. Sci Rep 11:7730 (2021). PubMed: 33833328
- Beasley AB et al. Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells. Methods Mol Biol 2265:213-222 (2021). PubMed: 33704717
- Graham M et al. Repeat domain-associated O-glycans govern PMEL fibrillar sheet architecture. Sci Rep 9:6101 (2019). PubMed: 30988362
- Yi WJ et al. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage. Cell Cycle 17:844-857 (2018). PubMed: 29623762
- McGill TJ et al. Allogeneic iPSC-Derived RPE Cell Graft Failure Following Transplantation Into the Subretinal Space in Nonhuman Primates. Invest Ophthalmol Vis Sci 59:1374-1383 (2018). Macaque monkey . PubMed: 29625461
- Regazzetti C et al. Melanocytes Sense Blue Light and Regulate Pigmentation through Opsin-3. J Invest Dermatol 138:171-178 (2018). PubMed: 28842328
- Zhou D et al. A novel P53/POMC/Gas/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation. J Cell Mol Med 21:802-815 (2017). WB, IHC-P ; Human . PubMed: 27885802
- McGill TJ et al. Long-Term Efficacy of GMP Grade Xeno-Free hESC-Derived RPE Cells Following Transplantation. Transl Vis Sci Technol 6:17 (2017). IHC-P ; Rat . PubMed: 28626601