Anti-Melanoma 抗体 [HMB45 + M2-7C10 + M2-9E3] (ab732)
製品の概要
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製品名Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3]
Melanoma 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [HMB45 + M2-7C10 + M2-9E3] to Melanoma
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由来種Mouse
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アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Frmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein
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エピトープHMB45 and MART-1
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ポジティブ･コントロール
- IHC-P: Metastatic melanoma in lymph node.
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特記事項
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. This cocktail is HMB45 + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, 99% Water -
精製度Tissue culture supernatant
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一次抗体 備考HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
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ポリ/モノモノクローナル
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クローン名HMB45 + M2-7C10 + M2-9E3
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ミエローマunknown
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アイソタイプIgG2b
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| Flow Cyt | Use at an assay dependent concentration. PubMed: 22147606 ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/100 - 1/200. The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. |
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| IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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関連性Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
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細胞内局在Membrane; Single-pass membrane protein
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参照データベース
- Entrez Gene: 22173 Mouse
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Entrez Gene: 308800 Rat
- Omim: 605513 Human
- Omim: 606933 Human
- SwissProt: P14679 Human
- SwissProt: Q16655 Human
see all -
別名
- Antigen LB39 AA antibody
- Antigen SK29 AA antibody
- MART1 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)](https://www.abcam.co.jp/ps/products/0/ab732/Images/ab732-292896-ab732.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)ab732 staining Melanoma in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)](https://www.abcam.co.jp/ps/products/0/ab732/Images/ab732_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma antibody [HMB45 + M2-7C10 + M2-9E3] (ab732)This image is courtesy of an anonymous Abreviewab732 staining murine melanoma metastasis tissue sections by IHC-P. Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated for 18 hours at 4°C. An Alexa Fluor 488® conjugated goat anti-mouse was used as the secondary
Confocal image shows melanoma in magenta, vessels in red (Collagen Type IV, ab19808), and melanin in cyan. Tissue is perfusion-fixed 15µm cryostat sections mounted on slides (i.e., lightly fixed, but not paraffin embedded).
参考文献
This product has been referenced in:
- Han N et al. Enhanced IL-34 expression in Nivolumab-resistant metastatic melanoma. Inflamm Regen 38:3 (2018). Read more (PubMed: 29515691) »
- Kong X et al. Cancer drug addiction is relayed by an ERK2-dependent phenotype switch. Nature 550:270-274 (2017). Read more (PubMed: 28976960) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"