Anti-MelanA 抗体 [EP1422Y] - BSA and Azide free (ab271839)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)](https://www.abcam.co.jp/ps/products/271/ab271839/Images/ab271839-1-ab5106162947antimelanaantibodyep1422yimmunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1422Y] to MelanA - BSA and Azide free
- Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-MelanA antibody [EP1422Y] - BSA and Azide free
MelanA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1422Y] to MelanA - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Melanoma lysate or melanoma tissue.
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
ab271839 is the carrier-free version of ab51061. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数（KD 値）
KD = 1.27 x 10 -9 M Learn more about KD -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP1422Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
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| IHC-P |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. -
組織特異性
Expression is restricted to melanoma and melanocyte cell lines and retina. -
翻訳後修飾
Acylated. -
細胞内局在
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. - Information by UniProt
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参照データベース
- Entrez Gene: 2315 Human
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
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別名
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)
ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51061).
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![Flow Cytometry - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)](https://www.abcam.co.jp/ps/products/271/ab271839/Images/ab271839-4-ab51061195356antimelanaantibodyep1422yflowcytometry.jpg)
Flow Cytometry - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)Overlay histogram showing Malme-3M cells stained with ab51061 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51061, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51061).
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![OI-RD Scanning - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)](https://www.abcam.co.jp/ps/products/271/ab271839/Images/ab271839-3-ab5106162945antimelanaantibodyep1422y.jpg)
OI-RD Scanning - Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51061). -
![Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)](https://www.abcam.co.jp/ps/products/271/ab271839/Images/ab271839-5-benefits-of-recombinant-antibodies.png)
Anti-MelanA antibody [EP1422Y] - BSA and Azide free (ab271839)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab271839 は論文での使用が確認できていません。
