Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Sample
Human Tissue sections (adult skin)
Specification
adult skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Diluent: PBS-T+1%BSA
Dilution
1/20
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Positive: antibody is really specific (the picutre shows epidermal melanocytes), and gives a good signal.
Negative: need to use a low dilution
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Heiko Locher
Verified customer
投稿 Sep 20 2013