Anti-MelanA 抗体 [DT101 + BC199] (ab731)

Reviews (3)Q&A (1)References (13)

Key features and details

  • Mouse monoclonal [DT101 + BC199] to MelanA
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Dog, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-MelanA antibody [DT101 + BC199]
    MelanA 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [DT101 + BC199] to MelanA

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Dog, Human

  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ･コントロール

    • Melanoma

  • 特記事項

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Constituents: PBS, Tissue culture supernatant

    Proprietary preservative that is not sodium azide or thimerosal, protein carrier.
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    DT101 + BC199

  • ミエローマ

    unknown

  • アイソタイプ

    IgG2b

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.

ターゲット情報

  • 機能

    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

  • 組織特異性

    Expression is restricted to melanoma and melanocyte cell lines and retina.

  • 翻訳後修飾

    Acylated.

  • 細胞内局在

    Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Antigen LB39 AA antibody
    • Antigen LB39-AA antibody
    • Antigen SK29 AA antibody
    • Antigen SK29-AA antibody
    • MAR1_HUMAN antibody
    • MART 1 antibody
    • MART-1 antibody
    • MART1 antibody
    • Melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T cells 1 antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • MLAN A antibody
    • MLANA antibody
    • OTTHUMP00000021036 antibody
    • OTTHUMP00000021037 antibody
    • OTTHUMP00000021038 antibody
    • Protein Melan-A antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)

    Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.

参考文献 (13)

ab731 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab731 は 13 報の論文で使用されています。

  • Semba T  et al. Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma. Cancer Sci 109:1447-1454 (2018). PubMed: 29498146
  • Zeng H  et al. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell 34:56-68.e9 (2018). PubMed: 29990501
  • Schwochow Thalmann D  et al. The evolution of Sex-linked barring alleles in chickens involves both regulatory and coding changes in CDKN2A. PLoS Genet 13:e1006665 (2017). IHC-Fr ; Chicken . PubMed: 28388616
  • Cao J  et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). PubMed: 27918305
  • Mi W  et al. YEATS2 links histone acetylation to tumorigenesis of non-small cell lung cancer. Nat Commun 8:1088 (2017). PubMed: 29057918
View all Publications for this product

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-MelanA antibody [DT101 + BC199]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin, melanocytes)
Permeabilization
No
Specification
Skin, melanocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 27 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Sample
Human Tissue sections (adult skin)
Specification
adult skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Read More

Mr. Heiko Locher

Verified customer

投稿 Sep 20 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lymph node)
Specification
lymph node
Fixative
Acetone
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 May 01 2009

Question

Hello, I wondered if you could help me please. I am looking to stain human biopsy tissue sections taken from melanoma tumours. I am interested in tumour antigens gp100, MART-1 and tyrosinase. Do you have any antibodies to these tumour antigens suitable for immunohistochemistry use? The biopsy tissue sections can be formalin fixed or frozen. I notice that you have several MART-1 antibodies, could you recommend which may be best for my purposes. Could you also forward a protocol or the method, if possible, and let me know whether I will require a secondary Ab, and if so which you would recommend. Kind Regards, Claire

Read More
Answer

I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and “Technical Enquiries” both accessible via the green menu bar on the datasheet. Here you will find customer reviews and past questions and answers specifically relating to that product. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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