Anti-MelanA 抗体 [DT101 + BC199] (ab731)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)](https://www.abcam.co.jp/ps/products/0/ab731/Images/ab731-205305-anti-melana-antibody-dt101-bc199-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)")
Key features and details
- Mouse monoclonal [DT101 + BC199] to MelanA
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-MelanA antibody [DT101 + BC199]
MelanA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [DT101 + BC199] to MelanA -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Dog, Human -
免疫原
Recombinant full length protein corresponding to Human MelanA.
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ポジティブ･コントロール
- Melanoma
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特記事項
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: PBS, Tissue culture supernatant
Proprietary preservative that is not sodium azide or thimerosal, protein carrier. -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
DT101 + BC199 -
ミエローマ
unknown -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
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| IHC-Fr |
Use at an assay dependent concentration.
ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. |
| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method. |
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IHC-Fr
Use at an assay dependent concentration. ABC method.It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas. |
ターゲット情報
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機能
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. -
組織特異性
Expression is restricted to melanoma and melanocyte cell lines and retina. -
翻訳後修飾
Acylated. -
細胞内局在
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. - Information by UniProt
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参照データベース
- Entrez Gene: 403495 Dog
- Entrez Gene: 2315 Human
- Entrez Gene: 77836 Mouse
- Entrez Gene: 293890 Rat
- Omim: 605513 Human
- SwissProt: Q16655 Human
- Unigene: 154069 Human
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別名
- Antigen LB39 AA antibody
- Antigen LB39-AA antibody
- Antigen SK29 AA antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.
データシートおよび資料
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Datasheet download
参考文献 (15)
ab731 は 15 報の論文で使用されています。
- Jeong YM et al. 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants. Cells 10:N/A (2021). PubMed: 33800001
- Ishibashi T et al. Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. Biosci Trends 14:390-395 (2020). PubMed: 32963182
- Semba T et al. Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma. Cancer Sci 109:1447-1454 (2018). PubMed: 29498146
- Zeng H et al. Bi-allelic Loss of CDKN2A Initiates Melanoma Invasion via BRN2 Activation. Cancer Cell 34:56-68.e9 (2018). PubMed: 29990501
- Cao J et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). PubMed: 27918305
