Anti-MelanA 抗体 [A103] (ab785)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
  • Flow Cytometry - Anti-MelanA antibody [A103] (ab785)

Key features and details

  • Mouse monoclonal [A103] to MelanA
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MelanA antibody [A103]
    MelanA 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [A103] to MelanA

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein (Mouse).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • 精製度

    IgG fraction

  • ポリ/モノ

    モノクローナル

  • クローン名

    A103

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab785の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

  • 組織特異性

    Expression is restricted to melanoma and melanocyte cell lines and retina.

  • 翻訳後修飾

    Acylated.

  • 細胞内局在

    Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Antigen LB39 AA antibody
      • Antigen LB39-AA antibody
      • Antigen SK29 AA antibody
      • Antigen SK29-AA antibody
      • MAR1_HUMAN antibody
      • MART 1 antibody
      • MART-1 antibody
      • MART1 antibody
      • Melan A antibody
      • Melan A protein antibody
      • Melanoma antigen recognized by T cells 1 antibody
      • Melanoma antigen recognized by T-cells 1 antibody
      • MLAN A antibody
      • MLANA antibody
      • OTTHUMP00000021036 antibody
      • OTTHUMP00000021037 antibody
      • OTTHUMP00000021038 antibody
      • Protein Melan-A antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
      Formalin-fixed, paraffin-embeded human melanoma tissue stained for Melan A using ab785 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [A103] (ab785)
      IHC image of ab785 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab785, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    • Flow Cytometry - Anti-MelanA antibody [A103] (ab785)
      Flow Cytometry - Anti-MelanA antibody [A103] (ab785)
      Overlay histogram showing Malme-3 cells stained with ab785 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab785, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    参考文献 (4)

    ab785 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab785 は 4 報の論文で使用されています。

    • Zingg D  et al. EZH2-Mediated Primary Cilium Deconstruction Drives Metastatic Melanoma Formation. Cancer Cell 34:69-84.e14 (2018). PubMed: 30008323
    • Suzuki S  et al. The Origin and Role of Autophagy in the Formation of Cytoplasmic Granules in Canine Lingual Granular Cell Tumors. Vet Pathol N/A:N/A (2014). Dog . PubMed: 25161210
    • Civenni G  et al. Human CD271-Positive Melanoma Stem Cells Associated with Metastasis Establish Tumor Heterogeneity and Long-term Growth. Cancer Res 71:3098-109 (2011). IHC-P ; Human . PubMed: 21393506
    • Chen YT  et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci U S A 93:5915-9 (1996). PubMed: 8650193

    レビューと Q&A

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-MelanA antibody [A103]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Melanocytes isolated from human nevus)
    Permeabilization
    Yes - triton 100x- 0,5%
    Specification
    Melanocytes isolated from human nevus
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Cristina Penas Lago

    Verified customer

    投稿 Mar 06 2018

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