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リコンビナントRabMAb

Anti-MEF2A 抗体 [EPR1451] (ab109420)

Submit a review Q&A (6)References (5)
Western blot - Anti-MEF2A antibody [EPR1451] (ab109420)
  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420)
  • Anti-MEF2A antibody [EPR1451] (ab109420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1451] to MEF2A
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MEF2A antibody [EPR1451]
    MEF2A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1451] to MEF2A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab151761)

  • ポジティブ･コントロール

    • Human heart, Human skeletal muscle, HeLa and Saos-2 lysate; HeLa cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109420の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.Can be blocked with MEF2A peptide (ab151761).
ICC/IF
1/100 - 1/250.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.Can be blocked with MEF2A peptide (ab151761).
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.

  • 組織特異性

    Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

  • 関連疾患

    Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].

  • 配列類似性

    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.

  • 翻訳後修飾

    Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
    Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
    Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ADCAD1 antibody
    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
    • MEF2 antibody
    • MEF2A antibody
    • MEF2A_HUMAN antibody
    • Myocyte enhancer factor 2A antibody
    • Myocyte-specific enhancer factor 2A antibody
    • RSRFC4 antibody
    • RSRFC9 antibody
    • Serum response factor like protein 1 antibody
    • Serum response factor-like protein 1 antibody
    see all

画像

  • Western blot - Anti-MEF2A antibody [EPR1451] (ab109420)
    Western blot - Anti-MEF2A antibody [EPR1451] (ab109420)
    All lanes : Anti-MEF2A antibody [EPR1451] (ab109420) at 1/10000 dilution

    Lane 1 : Human heart lysate
    Lane 2 : Human skeletal muscle lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Saos-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420)
    Immunocytochemistry/ Immunofluorescence - Anti-MEF2A antibody [EPR1451] (ab109420)
    ab109420, at 1/100 dilution, staining MEF2A in HeLa cells by Immunohistochemistry.
  • Anti-MEF2A antibody [EPR1451] (ab109420)
    Anti-MEF2A antibody [EPR1451] (ab109420)

参考文献 (5)

ab109420 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109420 は 5 報の論文で使用されています。

  • Payne S  et al. Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart. Nat Commun 10:3276 (2019). PubMed: 31332177
  • Vangoor VR  et al. Antagonizing Increased miR-135a Levels at the Chronic Stage of Experimental TLE Reduces Spontaneous Recurrent Seizures. J Neurosci 39:5064-5079 (2019). PubMed: 31015341
  • Carmichael RE  et al. MEF2A regulates mGluR-dependent AMPA receptor trafficking independently of Arc/Arg3.1. Sci Rep 8:5263 (2018). PubMed: 29588465
  • Zhu B  et al. The transcription factor MEF2A plays a key role in the differentiation/maturation of rat neural stem cells into neurons. Biochem Biophys Res Commun 500:645-649 (2018). PubMed: 29678571
  • Sacilotto N  et al. MEF2 transcription factors are key regulators of sprouting angiogenesis. Genes Dev 30:2297-2309 (2016). PubMed: 27898394

レビューと Q&A

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レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Question

What is the best fixative recommended for ICC with this product? I have typically found 1% formalin to best conserve the morphology of these cells....however I have not had much success using this antibody with 1% formalin. Would you recommend using 4% PFA instead? This is the protocol I have used so far (mouse cells): Pretreat w/ 0.1% or 0.25% TritonX100 (have tried both), 0.25% appears to perhaps work slightly better, though results are VERY faint Blocking: 10% Goat serum Primary: 1:100 overnight 4C and have also tried 1:100 RT (73F) for 90min, in 1% goat serum Negative control: 1% goat serum only Secondary: Goat anti-Rabbit IgG conj. AF488 1:1000 RT 1 hour (a protocol for this secondary which has been used for other primary antibodies with success in our lab) Coverslip with Prolong Gold AntiFade w/ DAPI There is a difference between the negative control and the section which received primary...but it is EXTREMELY faint (to capture on camera it required >8sec exposure). Do you think this might be attributed to the fixative? I'm just at a loss for what to try next....would welcome any suggestions!

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Answer

As you have noted, fixation is often the cause of an antibody not working as expected in ICC. Abcam recommends to fix samples for ICC either in ice-cold methanol, acetone (1-10 min) or in 3-4% paraformaldehyde in PBS pH 7.4 for 15 min at room temperature. If you are using acetone or methanol you may skip any further permeablization steps. We also recommend to incubate cells with 1% BSA in PBST for 30 min to block unspecific binding of the antibodies and to use the BSA/PBST solution for incubation of the primary and secondary antibodies.

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Question

immunogen peptide available for ab109420 and ab32866?

Read More
Answer

Thank you for your patience.
I have now heard back from the lab:
For ab109420, the immunogen peptide has been added to the catalog as ab151761.
https://www.abcam.com/MEF2A-peptide-ab151761.html
For ab32866, the immunogen is not available for sale. The immunogen was a fragment of the protein made in E. coli. The bacterial expression plasmid might be available for sale, but I would need to look into that further, if you are interested in the plasmid. Please let me know.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

immunogen peptide available for ab109420 and ab32866?

Read More
Answer

Thank you for contacting us.
The immunogen for ab109420 will be available for sale and be added to the catalog shortly.
As for ab32866, I am still waiting to hear back from the lab.
I will be in touch with more detailed information as soon as I have it.
Thank you for your patience.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

The MW is 50 and 75. Do you think this is the protein of interest?

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Answer

Thank you for your email.

I would say that the band you are seeing are the right band as no other bands are on the blot and the MW is in the right range. Please keep in mind that thescale is logarithmic, not linear.

You could use a positive control alongside your mouse heart samples, such as Human heart, Human skeletal muscle, HeLa or Saos-2 cell lysates.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Attached is an image of my western blot. Please take a look at it and let me know what you think.

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Answer

Thank you for your reply and for sending the image.

What is the MW of the two marker bands between which you obtained the protein bands?

In general, the mouse protein has 3 isoforms (http://www.uniprot.org/uniprot/Q60929) and 3 cleavage sites which would shorten the protein if cleavage takes place. This could explain the 2 bands you are seeing.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

WB with mouse heart
block: 5% milk
Ab: 1/10'000
2nd: HRP, works well
sees band at slightly different band size

Read More
Answer

Thank you for contacting us.

Could you please reply to this email and attach your WB image so I can take a look at it?

Also, please let me know if you have any other specific questions.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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