Application
RIP
Immuno-precipitation step
Protein G
Detection step
Other
Sample
Mouse Cell lysate - whole cell (Neuroblastoma N2a cell)
Specification
Neuroblastoma N2a cell
Negative control
Immunoprecipitation without radioactive labeled DNA probe
Type
RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Duration of cross-linking step: 0 second(s)
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA and 50mM free DNA in TBS buffer
Additional data
Additional Notes
MeCP2-DNA complex shifted upon addition of MeCP2 (ab2828) antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 15 2014