Anti-ME1 抗体 (ab97445)
Key features and details
- Rabbit polyclonal to ME1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ME1 antibody
ME1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ME1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow
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免疫原
Recombinant fragment corresponding to a region within amino acids 161-489 of Human ME1 (NP_002386).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97445の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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組織特異性
Expressed in all tissues tested including liver, placenta and white adipose tissue. -
配列類似性
Belongs to the malic enzymes family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 4199 Human
- Entrez Gene: 17436 Mouse
- Entrez Gene: 24552 Rat
- Omim: 154250 Human
- SwissProt: P48163 Human
- SwissProt: P06801 Mouse
- SwissProt: P13697 Rat
- Unigene: 21160 Human
see all -
別名
- Cytosolic malic enzyme 1 antibody
- HUMNDME antibody
- Malate dehydrogenase antibody
see all
画像
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Western blot - Anti-ME1 antibody (ab97445)All lanes : Anti-ME1 antibody (ab97445) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 64 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (ab97445)Immunofluorescence analysis of ME1 in paraformaldehyde fixed HeLa, using ab97445 at a 1/200 dilution.
Lower image: merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab97445 は 21 報の論文で使用されています。
- Jiang Z et al. miR-885-5p Inhibits Invasion and Metastasis in Gastric Cancer by Targeting Malic Enzyme 1. DNA Cell Biol 40:694-705 (2021). PubMed: 33751897
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Li H et al. CMap analysis identifies Atractyloside as a potential drug candidate for type 2 diabetes based on integration of metabolomics and transcriptomics. J Cell Mol Med 24:7417-7426 (2020). PubMed: 32469143
- Shao C et al. Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis. Redox Biol 36:101685 (2020). PubMed: 32853879
- Ghergurovich JM et al. A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway. Nat Chem Biol 16:731-739 (2020). PubMed: 32393898