MDR Assay Kit (Fluorometric) (ab112142)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
MDR Assay Kit (Fluorometric)
MDR キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
全工程の試験時間
1h 0m -
種交差性
交差種: Mammals, Other species -
製品の概要
Multi drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP dependent drug efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug resistance associated protein (MRP1) are members of the ABC transporter family. Abcam's Fluorimetric MDR Assay Kits use a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Abcam's Fluorimetric MDR Assay Kits are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.
Visit our FAQs page for tips and troubleshooting. -
特記事項
Store ab112142 dessicated.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests Assay Buffer 1 x 10ml 1 x 100ml DMSO 1 x 100µl 1 x 300µl MDR Sensor 1 vial 1 vial -
研究分野
画像
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Effect of Cyclosporin A on the inhibition of P-gp pump in MCF7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 µM (measured with the kit) is similar to the value reported in the literature.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab112142 は 6 報の論文で使用されています。
- Ahn JH et al. Effect of Rumex Acetosa Extract, a Herbal Drug, on the Absorption of Fexofenadine. Pharmaceutics 12:N/A (2020). PubMed: 32545588
- He H et al. Perimitochondrial Enzymatic Self-Assembly for Selective Targeting the Mitochondria of Cancer Cells. ACS Nano 14:6947-6955 (2020). PubMed: 32383849
- Vascellari S et al. Cisplatin, glutathione and the third wheel: a copper-(1,10-phenanthroline) complex modulates cisplatin-GSH interactions from antagonism to synergism in cancer cells resistant to cisplatin. RSC Adv 9:5362-5376 (2019). PubMed: 35515894
- Wang X et al. Extracellular ATP, as an energy and phosphorylating molecule, induces different types of drug resistances in cancer cells through ATP internalization and intracellular ATP level increase. Oncotarget 8:87860-87877 (2017). PubMed: 29152126
- Wang S et al. Schisandrin B reverses doxorubicin resistance through inhibiting P-glycoprotein and promoting proteasome-mediated degradation of survivin. Sci Rep 7:8419 (2017). PubMed: 28827665
- Wang S et al. Evodiamine synergizes with doxorubicin in the treatment of chemoresistant human breast cancer without inhibiting P-glycoprotein. PLoS One 9:e97512 (2014). Functional Studies . PubMed: 24830744