Anti-MDM2 (phospho S186) 抗体 [2G2] (ab22710)
Key features and details
- Mouse monoclonal [2G2] to MDM2 (phospho S186)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MDM2 (phospho S186) antibody [2G2]
MDM2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2G2] to MDM2 (phospho S186) -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MDM2 aa 150-250 (phospho S186). Synthetic peptide: QRKRHKSpDSIS, corresponding to amino acids 180-190 of Human MDM2.
Database link: Q00987-1 -
ポジティブ･コントロール
- Breast carcinoma.
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特記事項
Phosphorylation at serine 166 and 186 is necessary for MDM2's translocation into the nucleus, thereby facilitating interaction with p53.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A/G purified -
一次抗体 備考
Phosphorylation at serine 166 and 186 is necessary for MDM2's translocation into the nucleus, thereby facilitating interaction with p53. -
ポリ/モノ
モノクローナル -
クローン名
2G2 -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. -
組織特異性
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. -
関連疾患
Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding. -
配列類似性
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
ドメイン
Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself. -
翻訳後修飾
Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it. -
細胞内局在
Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4193 Human
- Omim: 164785 Human
- SwissProt: Q00987 Human
- Unigene: 484551 Human
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別名
- ACTFS antibody
- Double minute 2 protein antibody
- E3 ubiquitin-protein ligase Mdm2 antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab22710 は 3 報の論文で使用されています。
- Zhao Y et al. Germacrone induces lung cancer cell apoptosis and cell cycle arrest via the Akt/MDM2/p53 signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33880579
- Zhang Y et al. Comparative transcriptome analysis reveals PERP upregulated during Salmonella Enteritidis challenge in laying ducks. J Cell Physiol 234:11330-11347 (2019). PubMed: 30478915
- Wei S et al. Lenalidomide promotes p53 degradation by inhibiting MDM2 auto-ubiquitination in myelodysplastic syndrome with chromosome 5q deletion. Oncogene : (2012). Human . PubMed: 22525275