Anti-MDM2 (phospho S166) 抗体 (ab131355)
Key features and details
- Rabbit polyclonal to MDM2 (phospho S166)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MDM2 (phospho S166) antibody
MDM2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MDM2 (phospho S166) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human MDM2 conjugated to keyhole limpet haemocyanin. Synthetic phosphopeptide conjugated to KLH, surrounding phosphorylation site of Serine 166 (
A-I-S(p)-E-T
) derived from Human MDM2 (NP_002383.2).
Database link: Q00987-1 -
ポジティブ・コントロール
- 293 cell extract treated with Hydroxyurea, HeLa cells, Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab131355 is purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab131355の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200.
|
特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 55 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. -
組織特異性
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues. -
関連疾患
Note=Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding. -
配列類似性
Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain. -
ドメイン
Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the heterooligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself. -
翻訳後修飾
Phosphorylated in response to ionizing radiation in an ATM-dependent manner.
Auto-ubiquitinated; which leads to proteasomal degradation. Deubiquitinated by USP2 leads to its accumulation and increases deubiquitinilation and degradation of p53/TP53. Deubiquitinated by USP7; leading to stabilize it. -
細胞内局在
Nucleus > nucleoplasm. Cytoplasm. Nucleus > nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4193 Human
- Entrez Gene: 17246 Mouse
- Omim: 164785 Human
- SwissProt: Q00987 Human
- SwissProt: P23804 Mouse
- Unigene: 484551 Human
- Unigene: 22670 Mouse
- Unigene: 447669 Mouse
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別名
- ACTFS antibody
- Double minute 2 protein antibody
- E3 ubiquitin-protein ligase Mdm2 antibody
see all
画像
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All lanes : Anti-MDM2 (phospho S166) antibody (ab131355) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with hydroxyurea
Predicted band size: 55 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MDM2 (phospho S166) with ab131355 at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
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Immunofluorescence of methanol-fixed HeLa cells staining MDM2 (phospho S166) with ab131355 at a dilution of 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab131355 は 5 報の論文で使用されています。
- Barrio E et al. Preconditioning-Activated AKT Controls Neuronal Tolerance to Ischemia through the MDM2-p53 Pathway. Int J Mol Sci 22:N/A (2021). PubMed: 34298892
- Fukui Y et al. Investigation of the molecular causes underlying physical abnormalities in Diamond-Blackfan anemia patients with RPL5 haploinsufficiency. Pathol Int 71:803-813 (2021). PubMed: 34587661
- Rehman A et al. The desmosomal cadherin desmoglein-3 acts as a keratinocyte anti-stress protein via suppression of p53. Cell Death Dis 10:750 (2019). PubMed: 31582719
- Wu DM et al. MircoRNA-1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1. J Cell Mol Med 22:4963-4974 (2018). PubMed: 30024092
- Lim EG et al. Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53-independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells. Mol Med Rep 17:2572-2580 (2018). PubMed: 29207130