Anti-MD2 抗体 (ab24182)
Key features and details
- Rabbit polyclonal to MD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MD2 antibody
MD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MD2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24182の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).
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| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P | (5) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS. -
翻訳後修飾
N-glycosylated; high-mannose. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 23643 Human
- Entrez Gene: 17087 Mouse
- Entrez Gene: 448830 Rat
- Omim: 605243 Human
- SwissProt: Q9Y6Y9 Human
- SwissProt: Q9JHF9 Mouse
- Unigene: 726603 Human
- Unigene: 116844 Mouse
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別名
- ESOP 1 antibody
- ESOP-1 antibody
- ESOP1 antibody
see all
画像
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Western blot - Anti-MD2 antibody (ab24182)This image is courtesy of an Abreview submitted by C. GuerriAll lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)
Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at room temperature. -
Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml + Daudi cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182) -
Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182) at 1 µg/ml
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse testis lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182)All lanes : Anti-MD2 antibody (ab24182)
All lanes : Rat spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Western blot - Anti-MD2 antibody (ab24182)Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)
Predicted band size: 18 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in Rat Spleen cells with ab24182 at 10 μg/mL in ICC/IF.
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Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (ab24182)Immunofluorescent analysis of MD2 in human spleen cells with ab24182 at 20 μg/mL in ICC/IF. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (ab24182)Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (31)
ab24182 は 31 報の論文で使用されています。
- Huang S et al. Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling. Aging (Albany NY) 13:4409-4427 (2021). PubMed: 33495414
- Zhang P et al. Soluble MD-2 and Heme in Sickle Cell Disease Plasma Promote Pro-Inflammatory Signaling in Endothelial Cells. Front Immunol 12:632709 (2021). PubMed: 33841413
- Bai C et al. Effect of High Calorie Diet on Intestinal Flora in LPS-Induced Pneumonia Rats. Sci Rep 10:1701 (2020). PubMed: 32015367
- Zhang C et al. Direct inhibition of the TLR4/MyD88 pathway by geniposide suppresses HIF-1a-independent VEGF expression and angiogenesis in hepatocellular carcinoma. Br J Pharmacol 177:3240-3257 (2020). PubMed: 32144747
- Li XX et al. Cryptotanshinone from Salvia miltiorrhiza Bunge (Danshen) inhibited inflammatory responses via TLR4/MyD88 signaling pathway. Chin Med 15:20 (2020). PubMed: 32158495
