Anti-MCPIP1 抗体 (ab97910)
Key features and details
- Rabbit polyclonal to MCPIP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MCPIP1 antibody
MCPIP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCPIP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 291-474 of Human MCPIP1 (NP_079355).
-
ポジティブ・コントロール
- WB: HEK-293T, A431,whole cell lysate. IF: HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 66 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 66 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Has RNase activity and selectively degrades specific target mRNA species. Modulates the immune response and inflammation by regulating the decay of specific mRNA molecules. Recognizes the 3'-untranslated region (UTR) of the mRNA for IL6, CALCR and IL12B. Required for normal decay of IL6 mRNA (By similarity). Triggers apoptosis and promotes angiogenesis in response to the binding of CCL2 to CCR2. Regulates expression of CDH12 and CHD19. -
配列類似性
Belongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 80149 Human
- Omim: 610562 Human
- SwissProt: Q5D1E8 Human
- Unigene: 656294 Human
-
別名
- dJ423B22.1 antibody
- MCP 1 treatment-induced protein antibody
- MCP-induced protein 1 antibody
see all
画像
-
All lanes : Anti-MCPIP1 antibody (ab97910) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 66 kDa7.5% SDS-PAGE
-
Immunofluorescence analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling MCPIP1 protein (green) using ab97910 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab97910 は 2 報の論文で使用されています。
- Jin Z et al. Tetramethylpyrazine Preserves the Integrity of Blood-Brain Barrier Associated With Upregulation of MCPIP1 in a Murine Model of Focal Ischemic Stroke. Front Pharmacol 12:710358 (2021). PubMed: 34393790
- Xue M et al. Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production. Biosci Rep 39:N/A (2019). PubMed: 31651935