Anti-MCP1 抗体 (ab9899)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse
- Isotype: unknown
製品の概要
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製品名
Anti-MCP1 antibody
MCP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Mouse -
免疫原
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特記事項
JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Can be blocked with Recombinant mouse MCP1 protein (ab9901). To detect MCP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Sandwich ELISA | Use at an assay dependent concentration. To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine MCP-1 (500-P113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
ターゲット情報
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機能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 20296 Mouse
- SwissProt: P10148 Mouse
- Unigene: 290320 Mouse
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別名
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
画像
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Sandwich ELISA - Anti-MCP1 antibody (ab9899)ab9899 detecting MCP1 by sandwich ELISA.
プロトコール
データシートおよび資料
参考文献 (1)
ab9899 は 1 報の論文で使用されています。
- Lv J et al. Amygdalin ameliorates the progression of atherosclerosis in LDL receptor-deficient mice. Mol Med Rep 16:8171-8179 (2017). PubMed: 28983592
