Anti-MCP1 抗体 (ab73680)
Key features and details
- Rabbit polyclonal to MCP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCP1 antibody
MCP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human MCP1 aa 50 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab73679) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following Human Whole Cell Lysates: HCT 116, HeLa
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 11 kDa). |
ターゲット情報
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機能
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6347 Human
- Omim: 158105 Human
- SwissProt: P13500 Human
- Unigene: 303649 Human
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別名
- C-C motif chemokine 2 antibody
- CCL2 antibody
- CCL2_HUMAN antibody
see all
画像
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All lanes : Anti-MCP1 antibody (ab73680) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa, 50 kDa (possible glycosylated form), 80 kDa. We are unsure as to the identity of these extra bands.
The expression profile observed is consistent with what has been described in the literature (e.g. PMID 12957789). We believe the 50kDa band is a glycosylated form of Human MCP1.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab73680 は 9 報の論文で使用されています。
- Yang Q et al. C-C motif chemokine ligand 2/C-C receptor 2 is associated with glioma recurrence and poor survival. Exp Ther Med 21:564 (2021). PubMed: 33850536
- Chu CY et al. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics 11:3624-3641 (2021). PubMed: 33664852
- Back J et al. Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction. Bone Res 9:43 (2021). PubMed: 34588427
- Zhang Q et al. Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages. Nat Commun 12:6867 (2021). PubMed: 34824203
- Tan X et al. Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-ß1-NOX4 Pathway. J Mol Neurosci N/A:N/A (2020). PubMed: 32495004
- Tang H et al. Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer. Front Oncol 10:564298 (2020). PubMed: 33330033
- Kobayashi K et al. Role of monocyte chemoattractant protein-1 in liver fibrosis with transient myeloproliferative disorder in down syndrome. Hepatol Commun 2:230-236 (2018). PubMed: 29507898
- Zabini D et al. Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients. Eur Respir J 44:951-62 (2014). IHC . PubMed: 25034560
- Tjomsland V et al. The desmoplastic stroma plays an essential role in the accumulation and modulation of infiltrated immune cells in pancreatic adenocarcinoma. Clin Dev Immunol 2011:212810 (2011). IHC-P ; Human . PubMed: 22190968