Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (injred spinal cord tissue)
Specification
injred spinal cord tissue
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/1000
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in frozen sections (IHC-Fr). This antibody has not been tested in this application before, and it is possible that it is not suitable for use. However, we would be unable to confirm this without further testing of the antibody in the application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. soo ryung jeong
Verified customer
投稿 Oct 01 2009