Anti-MCP1 抗体 (ab7202)

Reviews (2)Q&A (14)References (72)
Western blot - Anti-MCP1 antibody (ab7202)

    Key features and details

    • Rabbit polyclonal to MCP1
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-MCP1 antibody
      MCP1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to MCP1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat
      交差が予測される動物種: Mouse

    • 免疫原

      Recombinant full length protein corresponding to Rat MCP1.
      Database link: P14844

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab7202 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/2000. Predicted molecular weight: 18 kDa.

    ターゲット情報

    • 機能

      Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

    • 配列類似性

      Belongs to the intercrine beta (chemokine CC) family.

    • 翻訳後修飾

      Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • C-C motif chemokine 2 antibody
        • CCL2 antibody
        • CCL2_HUMAN antibody
        • Chemokine (C C motif) ligand 2 antibody
        • GDCF 2 antibody
        • GDCF-2 antibody
        • GDCF2 antibody
        • HC11 antibody
        • HSMCR30 antibody
        • JE antibody
        • MCAF antibody
        • MCP 1 antibody
        • MCP-1 antibody
        • MCP1 antibody
        • MGC9434 antibody
        • Monocyte chemoattractant protein 1 antibody
        • Monocyte chemotactic and activating factor antibody
        • Monocyte chemotactic protein 1 antibody
        • Monocyte secretory protein JE antibody
        • SCYA2 antibody
        • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
        • Small inducible cytokine A2 antibody
        • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
        • Small-inducible cytokine A2 antibody
        • SMC CF antibody
        • SMC-CF antibody
        • SMCCF antibody
        see all

      画像

      • Western blot - Anti-MCP1 antibody (ab7202)
        Western blot - Anti-MCP1 antibody (ab7202)
        All lanes : Anti-MCP1 antibody (ab7202)

        Lane 1 : LPS-stimulated RAW cell lysate
        Lane 2 : Rat MCP1-transfected RAW cell lysate

        Predicted band size: 18 kDa



        Western blot analysis of RAW cell lysates labelling MCP1 with ab7202.

      参考文献 (72)

      ab7202 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab7202 は 72 報の論文で使用されています。

      • Yao L  et al. Coreopsis tinctoria Nutt ameliorates high glucose-induced renal fibrosis and inflammation via the TGF-ß1/SMADS/AMPK/NF-?B pathways. BMC Complement Altern Med 19:14 (2019). PubMed: 30630477
      • Chen X  et al. Adropin protects against liver injury in nonalcoholic steatohepatitis via the Nrf2 mediated antioxidant capacity. Redox Biol 21:101068 (2019). PubMed: 30684890
      • Chen X  et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep 39:N/A (2019). PubMed: 30760630
      • Chen L  et al. Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis. Exp Mol Med 51:38 (2019). PubMed: 30918245
      • Shimba Y  et al. Skeletal Muscle-specific PGC-1a Overexpression Suppresses Atherosclerosis in Apolipoprotein E-Knockout Mice. Sci Rep 9:4077 (2019). PubMed: 30858489
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-10 of 16 Abreviews or Q&A

      Western blot abreview for Anti-MCP1 antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (liver)
      Loading amount
      30 µg
      Specification
      liver
      Treatment
      2mg/kg LPS 1h
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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      Abcam user community

      Verified customer

      投稿 Nov 16 2012

      Question

      I am a Ph.D. student doing research on the effects of obesity on the placental development. Unfortunately the antibodies of interest have not been tested in the uterus/placenta. I am writing to see if your company would willing to give us a small sample of the anti-IL-8 catalog # ab7747 and anti MCP-1 catalog # ab7202 to do a test by immunohistochemistry on paraffin embedded samples of the rat uterus/placenta. I would be willing to share my results to help promote this product.

      Read More
      Answer

      Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

      We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

      Read More

      Question

      Thanks for your kindly reply, the information of this product as follow:

      Lot: gr47175-3

      Po: 1051535

      And after I contacted with this customer, she would like to have a credit note, therefore, could you please help her to create a credit note?


      I deeply appreciate your great assistance : )

      Best regards

      Read More
      Answer

      Thank you for your note.



      This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab7202. For your information, the internal reference note for this credit is CN20561. You can use this credit in the near future for any of the products which are in the catalogue.



      I hope this helps and if I can assist further, please do not hesitate to contact me.

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      Question

      Dear abcam technical support team:

      This customer has purchase ab7202 (Anti-MCP1 antibody) and has conducted the WB several times with mouse sample.

      The results show no band; moreover, this customer also has used RAW 264.7 cells and stimulated with LPS as a positive control, but the results also shows no band,

      she thinks our antibody was lost its function already. Therefore, she would like to ask for your help to solve her problem,

      I also attached her data and experiment step with different antibody in this letter, please check this and could you please offer any help to this customer,

      thanks for your assistance. Best regards



      1. Order details:


      Batch number: Lot: GR47175-3

      Abcam product code: ab7202

      Antibody storage conditions (temperature/reconstitution etc) : -20 ℃ freezer




      2. Please describe the problem (high background, wrong band size, more bands, no band etc).

      NO band

      3. On what material are you testing the antibody in WB?


      Species: mouse

      What’s cell line or tissue: renal tissue

      Cell extract or Nuclear extract: cell extract

      Purified protein or Recombinant protein: purified protein




      3. The lysate


      How much protein was loaded: 150 μg

      What lysis buffer was used: RIPA



      What protease inhibitors were used: protease inhibitor cocktail, PMSF, NaF NaVO3

      What loading buffer was used: SDS sample buffer with protein dye

      Phosphatase inhibitors: none

      Did you heat the samples: temperature and time: 100℃, 5 min




      4. Electrophoresis/Gel conditions/ Transfer conditions


      Reducing or non reducing gel: reducing gel

      Reducing agent: 2-mercaptoethanol

      Gel percentage : 12%

      Transfer conditions: (Type of membrane, Protein transfer verified): PVDF membrane, protein transfer apparatus from ATTO corporation (Model: AE6675)




      5. Blocking conditions


      Buffer: PBS with 0.1 % Tween 20

      Blocking agent: milk, BSA, serum, what percentage: 3% BSA

      Incubation time: 60 min

      Incubation temperature: 25 ℃




      6. Primary Antibody


      Species: rabbit

      Reacts against: MCP-1



      At what dilution(s) have you tested this antibody: 1:1000

      What dilution buffer was used: 3% BSA in PBS with 0.1% of Tween 20

      Incubation time: 18 hours

      Incubation temperature: 4 ℃

      What washing steps were done: washing membrane with PBS with 0.1% of Tween 20 for 6 times, 5 minutes per each time




      7. Secondary Antibody


      Species: goat

      Reacts against: rabbit antibody

      At what dilution(s) have you tested this antibody: 1:3000

      Incubation time: 1 hour

      Wash steps: washing membrane with PBS with 0.1% of Tween 20 for 6 times, 5 minutes per each time

      Fluorochrome or enzyme conjugate: HRP conjugate

      Do you know whether the problems you are experiencing come from the secondary? NO




      8. Detection method
      ECl, ECl+, other detection method: ECL



      9. Did you apply positive and negative controls along with the samples? Please specify. Yes, the cell lysate of RAW264.7

      10. Optimization attempts


      How many times have you tried the Western? 2 times

      Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): NO

      Do you obtain the same results every time e.g. are background bands always in the same place? NO

      What steps have you altered? NO

      Read More
      Answer

      Thank you for your enquiry regarding ab7202 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

      The protocol looks fine to me. It may well be the antibody has been accidentally damaged.

      I can offer a new vial free of charge for your troubled customer but all our vials are from the same belled and purification. Could you please confirm the Abcam Order and the date of purchase and let me know how your customer would like to proceed with this enquiry.

      I look forward to hearing from you soon.

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      Question

      No signal in ELISA with ab20641. No signal in WB with ab7202.

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      Answer

      Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original orders and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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      Question

      Nuclear staining in mouse spleen and lymph node.

      Read More
      Answer

      Thank you for your call today and for your questions about ab7202. As we discussed, we recommend using a microwave antigen retrieval method with this antibody. However, if the antibody is still staining the wrong localization, please let me know and we will be happy to send a replacement or issue a credit or refund. I look forward to hearing from you. Let me know if you have any questions.

      Read More

      Immunohistochemistry (Frozen sections) abreview for Anti-MCP1 antibody

       Poor
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (injred spinal cord tissue)
      Specification
      injred spinal cord tissue
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Ms. soo ryung jeong

      Verified customer

      投稿 Oct 01 2009

      Question

      BATCH NUMBER 7052 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Western blot: no bands detectable Immunohistochemistry: no reaction SAMPLE WB: mouse aorta protein extract IHC: mouse frozen cross sections of the aorta, OCT embedded PRIMARY ANTIBODY WB: 1:2000, 1:1000, 1:500 over night, wash step 2x 5min in TBST 0.05% IH: over night 1:400, 1:200, 1:100, wash step 3x 5min in PBS SECONDARY ANTIBODY WB: Amersham donkey anti-rabbit IgG HRP, 1:3500, 60mion, wash step 1x 15min in TBST 0.05% IH: Vector/Linaris goat anti-rabbit IgG Biotin in PBS, 1:200, 45min or 60min, wash step 3x 5min PBS DETECTION METHOD WB: ECL IH: DAB-chromogen (Dako) POSITIVE AND NEGATIVE CONTROLS USED IH: positive: serial sections that showed positive staining with thsi primary asntibody, but different Lot (56165) negative: unspecific IgG SAMPLE PREPARATION WB: RIPA buffer + protease inhibitor (complete, mini EDTA-free, Roche) 10:1 loading buffer RotiLoad 1 (Roth) 1:4, 95?C, 5min TRANSFER AND BLOCKING CONDITIONS WB: transfer: transfer buffer (25mM Tris base, 0.2M glycine, 20% methanol, pH 8.5, 60min 340mA or transfer over night 90mA block: 5% milk/TBST, 1h IH: block: 6% goat serum in 4% BSA/PBS or 4% goat serum HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4both HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? WB: first antibody concentration, blocking concentration as indicated IH: first antibody concentration, incubation period, blocking agents as indicated, changed other reagents and tested them iwth another primary antibody in order to find out whether they are still working (positive results).

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      Answer

      I'm sorry to hear the customer is having problems with his new vial of ab7202. Thank you for taking the time to fill in the questionnaire, I can see clearly that the protocol looks very good and the antibody should perform if the customer has previously had good results. Could you please confirm the lot number or the order number for this vial as we have no record of a lot 7052 and confirm the storage conditions, my apologies for the inconvenience. Could you please also send us an image of the positive staining with lot 56165? Thank you for your patience, we look forward to hearing from you soon,

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      Question

      BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 57806 DESCRIPTION OF THE PROBLEM low signal and high background using a biotinylated secundary antibody non-signal using a FITC-conjugated secondary antibody SAMPLE Aortic frozen sections from mice (apo E-/- and LDL-/- PRIMARY ANTIBODY Rabbit polyclonal to MCP-1 (ab7202, Abcam)incubated a)for 2-3 hours at room temperature b)overnight at 4?C Dilutions used: 1/25, 1/50, 1/100, 1/500, 1/1000 Wash step: Rinse 2x5 min in PBS SECONDARY ANTIBODY a)Biotinylated goat anti-rabbit (Vector) Incubation at room temperature for 2-3 h. Dilution used: 1/200 (in BSA 1%) b)FITC-goat anti rabbit (Alexa Fluor) Incubation at room temperature for 3 h. Dilution used: 1/300 DETECTION METHOD a)ABC complex (Vector): used in a 1/200 dilution and incubated for 45 min at room temperature.Developed with DAB chromogen at room temperature,counterstained with haematoxylin, dehydrated and mounted in DPX. b)Mounted in Mowiol. POSITIVE AND NEGATIVE CONTROLS USED Positive controls : aorta from mice (32 and 24 weeks of age) fed with a high-cholesterol diet for either 22 or 14 weeks. Negative controls: the same tissue without adding the primary antibody. ANTIBODY STORAGE CONDITIONS 4?C FIXATION OF SAMPLE Acetone for 5 min ANTIGEN RETRIEVAL None PERMEABILIZATION STEP Sodium deoxycholate for 10 min 0.5 % Triton in PBS solution for 10 min BLOCKING CONDITIONS 1%BSA in PBS for 30 min at room temperature avidin/biotin for 10 min each at room temperature. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES I would like to know whether someone has already tried this antibody in aorta from mice.

      Read More
      Answer

      I'm sorry to hear you are having problem with ab7102. We have not tested it in mouse aorta or have any reviews yet from customers using it in aorta. So please do let us know when your staining works and we will reward you with an Amazon gift voucher (a review can be submitted via our online datasheet). I would like to suggest the following modifications to your protocol: -wash between all steps minimum 4 times 5min in PBS -fix for 10min in methanol or acetone pre-cooled in the freezer -block endogenous peroxidases with hydrogen peroxide (0.3% v/v in PBST) -block the slides with normal goat serum 10% v/v 1hr -use PBST (0.1-0.5%v/v triton) to dilute the serum, the primary and secondary antibody. -Incubate the biotin antibody for 2 hours at 1:400 in PBST, no BSA. -the dilution of the detection kit from Vector can be altered to decrease the background, make sure you prepare the ABC in PBS and leave to rest for 30min before applying onto slides. Try 1:500 for 1hr. It is best to leave it longer but more dilute. -When washing the slides after the DAB reaction, use Tris buffer 0.5M rather than PBS. This will decrease background as well. If you want to use the FITC-conjugated antibody dilute it in PBST (pH 7 or 8). For background problems please always check the secondary antibodies are not responsible. To do so, do the same protocol simply omitting the primary antibody. Please let me know if this helps and do not hesitate to contact us for further advice,

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      Question

      Customer called about what of fixative to use for IHC-Frozen sections.

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      Answer

      Thank you for your phone call. There are no special conditions that need to be followed to use ab7202 in IHC-frozen sections and so I suggest fixing your sections with acetone as per our general IHC protocol for frozen sections (the protocol can be found in the protocols tab of the online datasheet).

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      1-10 of 16 Abreviews or Q&A

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