Key features and details
- Mouse monoclonal [47DC141] to MCM7/PRL
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Xenopus laevis
- Isotype: IgG1
製品名Anti-MCM7/PRL antibody [47DC141]
MCM7/PRL 一次抗体 製品一覧
製品の詳細Mouse monoclonal [47DC141] to MCM7/PRL
アプリケーション適用あり: IHC-P, Flow Cyt, ICC/IFmore details
種交差性交差種: Mouse, Rat, Dog, Human, Xenopus laevis
Recombinant full length protein corresponding to Human MCM7/PRL.
- Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
This product was previously labelled as MCM7
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab2360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
配列類似性Belongs to the MCM family.
Contains 1 MCM domain.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
This image shows immunostaining of MCM7/PRL in rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody MCM7/PRL (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
ab2360 staining MCM7/PRL in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted.
ICC/IF image of MCM7/PRL (ab2360) stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLA cells stained with MCM7/PRL (ab2360) (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2360, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab2360 staining MCM7/PRL in formalin-fixed, paraffin-embedded Human breast carcinoma tissue tissue by Immunohistochemistry.
ab2360 は 23 報の論文で使用されています。
- Kovary KM et al. Expression variation and covariation impair analog and enable binary signaling control. Mol Syst Biol 14:e7997 (2018). PubMed: 29759982
- Drissi R et al. Destabilization of the MiniChromosome Maintenance (MCM) complex modulates the cellular response to DNA double strand breaks. Cell Cycle 17:2593-2609 (2018). PubMed: 30516086
- Charrasse S et al. Ensa controls S-phase length by modulating Treslin levels. Nat Commun 8:206 (2017). PubMed: 28785014
- Bai G et al. Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation. PLoS Genet 12:e1005787 (2016). WB ; Mouse . PubMed: 26765334
- Guan B et al. Minichromosome maintenance complex component 7 has an important role in the invasion of papillary urothelial neoplasia. Oncol Lett 10:946-950 (2015). PubMed: 26622601