Anti-MCM6 抗体 (ab4458)
Key features and details
- Rabbit polyclonal to MCM6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM6 antibody
MCM6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Binds to chromatin during G1 and detach from it during S phase. - Information by UniProt
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参照データベース
- Entrez Gene: 517812 Cow
- Entrez Gene: 4175 Human
- Entrez Gene: 29685 Rat
- Omim: 601806 Human
- SwissProt: Q2KIZ8 Cow
- SwissProt: Q14566 Human
- SwissProt: Q62724 Rat
- Unigene: 444118 Human
see all -
別名
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-MCM6 antibody (ab4458)Sample: HeLa cell RIPA extract (50
µ g).Antibody: ab4458 at 0.2
Sample: HeLa cell RIPA extract (50 µg).µ g/ml incubated for 1 hour.
Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
参考文献 (5)
ab4458 は 5 報の論文で使用されています。
- Chen QY et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213
- Quimbaya M et al. Deregulation of the Replisome Factor MCMBP Prompts Oncogenesis in Colorectal Carcinomas through Chromosomal Instability. Neoplasia 16:694-709 (2014). Human . PubMed: 25246271
- Gronnier C et al. The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells. Biochim Biophys Acta 1843:2432-7 (2014). PubMed: 25003315
- Kawabata T et al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB ; Mouse . PubMed: 21611832
- Takahashi N et al. The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. PLoS Genet 6:e1000817 (2010). WB ; Human . PubMed: 20090939
