Anti-MCM5 抗体 [EP2683Y] (ab75975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2683Y] to MCM5
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MCM5 antibody [EP2683Y]
MCM5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2683Y] to MCM5 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, IP, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: MCF7 cell lysate. Mouse spleen lysate; IHC-P: Human tonsil tissue; Flow Cyt (intra): MCF7 cells; ICC/IF: MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2683Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75975の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/50.
For unpurified use at 1/100 - 1/250. |
IP |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
For unpurified use at 1/1000 - 1/10000. |
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IHC-P |
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
特記事項 |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250. |
IP
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). For unpurified use at 1/1000 - 1/10000. |
IHC-P
1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/250 - 1/500. |
ターゲット情報
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配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4174 Human
- Entrez Gene: 17218 Mouse
- Omim: 602696 Human
- SwissProt: P33992 Human
- SwissProt: P49718 Mouse
- Unigene: 517582 Human
- Unigene: 5048 Mouse
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別名
- CDC 46 antibody
- CDC46 antibody
- CDC46 homolog antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling MCM5 with purified ab75975 at 1/1000 dilution (0.11 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-MCM5 antibody [EP2683Y] (ab75975) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : Mouse spleen lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 82 kDaBlocking Buffer and concentration: 5% NFDM/TBST.
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Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1/20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1:50 dilution (2.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab75975 は 13 報の論文で使用されています。
- Yap K et al. Hybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments. Mol Cell 82:463-478.e11 (2022). PubMed: 34741808
- Zacarías-Fluck MF et al. The Wnt signaling receptor Fzd9 is essential for Myc-driven tumorigenesis in pancreatic islets. Life Sci Alliance 4:N/A (2021). PubMed: 33653688
- Zhang LL et al. MCM5 Aggravates the HDAC1-Mediated Malignant Progression of Lung Cancer. Front Cell Dev Biol 9:669132 (2021). PubMed: 34409025
- Lei Y et al. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments. Life Sci 272:119227 (2021). PubMed: 33607151
- Cao T et al. Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma. Biomed Res Int 2020:3574261 (2020). PubMed: 32964028