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  1. Link

    mcm4-antibody-ab4459.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Other
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Anti-MCM4 抗体 (ab4459)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (27)

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Abpromise

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Western blot - Anti-MCM4 antibody (ab4459)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
  • Western blot - Anti-MCM4 antibody (ab4459)
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)

Key features and details

  • Rabbit polyclonal to MCM4
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human, Xenopus laevis
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-MCM4 antibody [EPR5245] (ab124836)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-MCM4 antibody
    MCM4 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to MCM4
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details
    適用なし: IP
  • 種交差性

    交差種: Mouse, Human, Xenopus laevis
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • 免疫原

    Synthetic peptide within Human MCM4 aa 750-850. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005905.2 (GeneID 4173).
    Database link: P33991

  • ポジティブ・コントロール

    • IHC-P: Human testicular seminoma and mouse squamous cell carcinoma tissue. Human testis tissue. WB: HeLa cell RIPA extract. HeLa nuclear cell lysate. ICC: HeLa cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4459の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa).
特記事項
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 96.6 kDa).
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
  • 配列類似性

    Belongs to the MCM family.
    Contains 1 MCM domain.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P33991 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 35679 Drosophila melanogaster
    • Entrez Gene: 4173 Human
    • Entrez Gene: 17217 Mouse
    • Omim: 602638 Human
    • SwissProt: Q26454 Drosophila melanogaster
    • SwissProt: P33991 Human
    • SwissProt: P49717 Mouse
    • Unigene: 21562 Drosophila melanogaster
    • Unigene: 460184 Human
    • Unigene: 1500 Mouse
    see all
  • 別名

    • 19G antibody
    • AI325074 antibody
    • AU045576 antibody
    • CDC 21 antibody
    • CDC 54 antibody
    • CDC21 antibody
    • CDC21 homolog antibody
    • CDC21, S. pombe, homolog of antibody
    • CDC54 antibody
    • Cell division cycle 21, S. pombe, homolog of antibody
    • DNA replication licensing factor MCM 4 antibody
    • DNA replication licensing factor MCM4 antibody
    • hCdc 21 antibody
    • hCdc21 antibody
    • Homolog of S. pombe cell devision cycle 21 antibody
    • KIAA4003 antibody
    • mcdc21 antibody
    • MCM 4 antibody
    • MCM 4 minichromosome maintenance deficient 4 antibody
    • mcm4 antibody
    • MCM4 minichromosome maintenance deficient 4 antibody
    • MCM4 minichromosome maintenance deficient 4, mitotin (S. cerevisiae) antibody
    • MCM4_HUMAN antibody
    • Mcmd4 antibody
    • MGC33310 antibody
    • Minichromosome maintenance 4 antibody
    • Minichromosome maintenance complex component 4 antibody
    • Minichromosome maintenance deficient (S. cerevisiae) 4 antibody
    • Minichromosome maintenance deficient 4 antibody
    • Minichromosome maintenance deficient 4 homolog (S. cerevisiae) antibody
    • Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody
    • mKIAA4003 antibody
    • NKCD antibody
    • NKGCD antibody
    • P1 CDC21 antibody
    • P1-CDC21 antibody
    • P1CDC21 antibody
    see all

画像

  • Western blot - Anti-MCM4 antibody (ab4459)
    Western blot - Anti-MCM4 antibody (ab4459)
    HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)

    IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-MCM4 antibody (ab4459)
    Western blot - Anti-MCM4 antibody (ab4459)
    Anti-MCM4 antibody (ab4459) at 1/2000 dilution + HeLa nuclear cell lysate. at 20 µg

    Secondary
    Goat anti rabbit HRP. at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 96.6 kDa



    This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image.
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)
    Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)
    ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (27)

ab4459 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4459 は 27 報の論文で使用されています。

  • Kim D  et al. Arsenic hexoxide has differential effects on cell proliferation and genome-wide gene expression in human primary mammary epithelial and MCF7 cells. Sci Rep 11:3761 (2021). PubMed: 33580144
  • Rainey MD  et al. ATR Restrains DNA Synthesis and Mitotic Catastrophe in Response to CDC7 Inhibition. Cell Rep 32:108096 (2020). PubMed: 32877678
  • Prendergast L  et al. Histone chaperone FACT is essential to overcome replication stress in mammalian cells. Oncogene 39:5124-5137 (2020). PubMed: 32533099
  • Hashimoto Y & Tanaka H Ongoing replication forks delay nuclear envelope breakdown upon mitotic entry. J Biol Chem N/A:N/A (2020). PubMed: 33148697
  • Cao T  et al. Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma. Biomed Res Int 2020:3574261 (2020). PubMed: 32964028
View all Publications for this product

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-MCM4 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

投稿 Aug 22 2005

Question

Hi,    I;m a graduate student from University of Southern California. Our lab works with Pombe/ fission yeast. I;m interested in using the mcm4 antibody (ab 4459) to see if it works with pombe. I would like to find out if there is any way that I can determine if the above mentioned antibody could be used in our system.Further if there if it will be possible to obtain a sample of the antibody to test before making a purchase    thanks

Read More

Abcam community

Verified customer

Asked on Nov 18 2011

Answer

Thank you very much for your inquiry. I am sorry to confirm that I ran a blast search and could not find any homology information of the immunogen used to generate this antibody and Schizosaccharomyces pombe. The homology to Saccharomyces cerevisiae is only 50% and therefore I assume that this antibody is also not likely to cross-react with Schizosaccharomyces pombe. We expect cross-reactivity from 85% upwards. I am sorry I do not have a positive answer to your inquiry on this occasion and hope this information is nevertheless helpful.

Read More

Abcam Scientific Support

Answered on Nov 18 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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