Anti-MCM4 抗体 (ab3728)
Key features and details
- Goat polyclonal to MCM4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM4 antibody
MCM4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MCM4 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.
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ポジティブ･コントロール
- WB: HeLa, HEK293T, Jurkat cell lysate. IHC-P: human ovarian carcinoma, mouse renal cell carcinoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3728の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
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| 特記事項 |
|---|
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
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別名
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
画像
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Western blot - Anti-MCM4 antibody (ab3728)All lanes : Anti-MCM4 antibody (ab3728) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 100 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab3728 は 4 報の論文で使用されています。
- Lv Q et al. Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3ß/ß-catenin and TGF-ß1/Smad2/3 signaling pathways. Mol Med 26:49 (2020). PubMed: 32448163
- Zhang L et al. LRP6 is involved in the proliferation, migration and invasion of trophoblast cells via miR-346. Int J Mol Med 46:211-223 (2020). PubMed: 32319541
- Tenca P et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007). PubMed: 17062569
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human, Chicken . PubMed: 17635936