Anti-MCM4 抗体 (ab3728)
Key features and details
- Goat polyclonal to MCM4
- Suitable for: IHC-P, WB
- Reacts with: Chicken, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MCM4 antibody
MCM4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MCM4 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Chicken, Human
交差が予測される動物種: Mouse, Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 4 encoded in part by exons 8 and 9.
-
ポジティブ･コントロール
- HeLa cell lysate.
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa). |
ターゲット情報
-
機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 4173 Human
- Entrez Gene: 17217 Mouse
- Omim: 602638 Human
- SwissProt: P33991 Human
- SwissProt: P49717 Mouse
- Unigene: 460184 Human
- Unigene: 1500 Mouse
-
別名
- 19G antibody
- AI325074 antibody
- AU045576 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab3728)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MCM4 with ab3728 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-MCM4 antibody (ab3728)Sample: Hela cell RIPA extract (60
µ g)Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60
µg) Antibody: Affinity purified Goat anti-MCM 4 (ab3728) diluted 1 / 4000.
Detection: ECL.
プロトコール
参考文献 (3)
ab3728 は 3 報の論文で使用されています。
- Lv Q et al. Pirfenidone alleviates pulmonary fibrosis in vitro and in vivo through regulating Wnt/GSK-3ß/ß-catenin and TGF-ß1/Smad2/3 signaling pathways. Mol Med 26:49 (2020). PubMed: 32448163
- Tenca P et al. Cdc7 is an active kinase in human cancer cells undergoing replication stress. J Biol Chem 282:208-15 (2007). PubMed: 17062569
- Hochegger H et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol 178:257-68 (2007). WB ; Human, Chicken . PubMed: 17635936
