Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
製品名Anti-MCM3 antibody [EPR7080] - BSA and Azide free
MCM3 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7080] to MCM3 - BSA and Azide free
アプリケーション適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MCM3. The exact sequence is proprietary.
Database link: P25205
Ab248222 is the carrier-free version of ab128923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
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Our Abpromise guarantee covers the use of ab248222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
配列類似性Belongs to the MCM family.
Contains 1 MCM domain.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Cervical cancer proto oncogene 5 antibody
- DNA polymerase alpha holoenzyme associated P1 antibody
- DNA polymerase alpha holoenzyme associated protein P1 antibody
This data was developed using ab128923, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.