Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4119] to MCM2 (phospho S27) - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
製品名Anti-MCM2 (phospho S27) antibody [EPR4119] - BSA and Azide free
MCM2 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR4119] to MCM2 (phospho S27) - BSA and Azide free
This antibody only detects MCM2 when phosphorylated at serine 27.
アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MCM2 (phospho S27). The exact sequence is proprietary.
Ab247872 is the carrier-free version of ab109459. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247872 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab247872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.|
|IP||Use at an assay dependent concentration.|
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
配列類似性Belongs to the MCM family.
Contains 1 MCM domain.
翻訳後修飾Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Information by UniProt
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
This data was developed using ab109459, the same antibody clone in a different buffer formulation.
ab109459 at 1/100 dilution staining MCM2 (phospho S27) in paraffin-embedded Human tonsil tissue by Immunohistochemistry.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.