Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~110 kDa
Positive control
uninfected cells were used as a negative control
Additional data
Additional Notes
great antibody worked great with our experiments.
HeLa cell nuclear extracts were used.
HeLa cell nuclear extracts were used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 21 2005