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Anti-MCM2 抗体 [CRCT2.1 (D1.9H5)] (ab6153)

  • Datasheet
  • SDS
Reviews (7)Q&A (3)References (10)

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Abpromise

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Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)

    Key features and details

    • Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    こちらの製品もご検討ください

    二次抗体
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    関連製品

    製品の概要

    • 製品名

      Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]
      MCM2 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
    • 由来種

      Mouse
    • 特異性

      Recognises human Mcm2
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Recombinant full length protein corresponding to MCM2. Bacterially expressed human Mcm2.

    • ポジティブ・コントロール

      • Tonsil, any tissue with high proliferative index
    • 特記事項

      Clone Number: CRCT2.1 (D1.9H5)

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 特記事項(精製)

      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • ポリ/モノ

      モノクローナル
    • クローン名

      CRCT2.1 (D1.9H5)
    • ミエローマ

      Sp2
    • アイソタイプ

      IgG1
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • Other

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    Our Abpromise guarantee covers the use of ab6153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).

    ターゲット情報

    • 機能

      Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
    • 配列類似性

      Belongs to the MCM family.
      Contains 1 MCM domain.
    • 翻訳後修飾

      Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
    • 細胞内局在

      Nucleus.
    • Target information above from: UniProt accession P49736 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 4171 Human
      • Omim: 116945 Human
      • SwissProt: P49736 Human
      • Unigene: 477481 Human
      • 別名

        • BM28 antibody
        • CCNL 1 antibody
        • CCNL1 antibody
        • CDC like 1 antibody
        • cdc19 antibody
        • CDCL 1 antibody
        • CDCL1 antibody
        • Cell devision cycle like 1 antibody
        • Cyclin like 1 antibody
        • D3S3194 antibody
        • DNA replication licensing factor MCM2 antibody
        • KIAA0030 antibody
        • MCM 2 antibody
        • MCM2 antibody
        • MCM2 minichromosome maintenance deficient 2 mitotin antibody
        • MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae) antibody
        • MCM2 minichromosome maintenance deficient 2, mitotin antibody
        • MCM2_HUMAN antibody
        • MGC10606 antibody
        • Minichromosome maintenance complex component 2 antibody
        • Minichromosome maintenance deficient 2 (mitotin) antibody
        • Minichromosome maintenance deficient 2 mitotin antibody
        • Minichromosome maintenance protein 2 antibody
        • Minichromosome maintenance protein 2 homolog antibody
        • Mitotin antibody
        • Nuclear protein BM28 antibody
        • OTTHUMP00000216047 antibody
        • OTTHUMP00000216050 antibody
        see all

      画像

      • Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)
        Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)
        Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

        Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.

      プロトコール

      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
      • SDS
    • 参考文献 (10)

      ab6153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6153 は 10 報の論文で使用されています。

      • Wienert B  et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun 11:2109 (2020). PubMed: 32355159
      • Mishra S  et al. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell 174:803-817.e16 (2018). PubMed: 30057114
      • Scapulatempo-Neto C  et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . PubMed: 28454340
      • Zhang AT  et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). PubMed: 21610089
      • Shtutman M  et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . PubMed: 20368428
      • Sarfraz S  et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . PubMed: 18680610
      • Villadsen R  et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol 177:87-101 (2007). PubMed: 17420292
      • Niklaus AL  et al. Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. Hum Reprod 22:1778-88 (2007). PubMed: 17371803
      • Canela N  et al. Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A. J Biol Chem 281:35942-53 (2006). WB ; Human . PubMed: 17001081
      • Duursma A & Agami R p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation. Mol Cell Biol 25:6937-47 (2005). WB . PubMed: 16055707

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 10 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Kidney biopsies)
      Specification
      Kidney biopsies
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 19 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Stomach)
      Specification
      Stomach
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 30 2012

      Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK293T)
      Loading amount
      20 µg
      Specification
      HEK293T
      Gel Running Conditions
      Reduced Denaturing (8% SDS-PAGE)
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 30 2012

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver biopsy)
      Specification
      Liver biopsy
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Read More

      Dr. Saira Khalid

      Verified customer

      投稿 Apr 07 2010

      Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - other (HeLa)
      Loading amount
      100 µg
      Specification
      HeLa
      Gel Running Conditions
      Reduced Denaturing (8% gel)
      Blocking step
      Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      投稿 Dec 18 2008

      Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - nuclear (SupT1, Jurkat, Hela cells)
      Loading amount
      15 µg
      Specification
      SupT1, Jurkat, Hela cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 08 2006

      Question

      A customer needs an ab to human MCM2 for IHC and IF. The closest I found is ab6153, but it was not tested for IF. Would you expect it to be applicable for IF? (We know that the abpromise will not be valid for IF).

      Read More

      Abcam community

      Verified customer

      Asked on Jul 16 2006

      Answer

      The antibody has good chances of working in other types of immunohistochemistry other than those already tested as it has the ability to recognise the native form of the protein. If the customer means IF as in ICC/IF I would recommend to try fixing the cells in PFA 5-10min. If the customer means IF as in IHC-P or IHC-Fr with an IF detection method I would recommend to follow our recommendation for those methods but use an IF amplification kit (i.e either a biotin secondary + extra-avidin/FITC or TRITC method or more sensitive methods which come from Kits eg the TSA kit). I hope this will help your customer,

      Read More

      Abcam Scientific Support

      Answered on Jul 17 2006

      Western blot abreview for Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - nuclear (HeLa)
      Specification
      HeLa
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 21 2005

      Question

      I am going to buy your products, MCM2 antibody. But I found several same antibody from your company. This antibody will be used in Western blot. Could you tell me what difference between them(such as, ab1896, ab6153,ab4461)? Are they all antibody for phosphoMCM2? Could you help me to decide which I had better to choose? Many thanks

      Read More

      Abcam community

      Verified customer

      Asked on Nov 04 2004

      Answer

      All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain their level of usefulness in your experimental procedures. I'm sorry for not being able to give you more guidance. If you require additional information to that provided on the product datasheet, please contact us again and we will gladly assist you.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2004

      Question

      I need to know it's success rate for immunhistochemistry on formalin fixed, paraffin embedded tissues.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 14 2002

      Answer

      All the information we have for this product for use in IHC is on the datasheet, including recommended dilutions and advice for antigen retrieval. I also suggest that you click through to the review submitted by a researcher who successfully used this antibody in IHC on human lung cancer paraffin embedded tissue. In terms of the "success rate" this will depend on a number variables, including the species that you are studying. Species cross-reactivity for this product has not been tested. However, as discussed this antibody works very well in IHC on human tissue.

      Read More

      Abcam Scientific Support

      Answered on Jan 15 2002

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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