Anti-MCM2 抗体 [CRCT2.1 (D1.9H5)] (ab6153)
![Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)](https://www.abcam.co.jp/ps/products/6/ab6153/Images/ab6153-12068-anti-mcm2-antibody-crct21-d19h5-western-blot.jpg "Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)")
Key features and details
- Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-MCM2 antibody [CRCT2.1 (D1.9H5)]
MCM2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CRCT2.1 (D1.9H5)] to MCM2 -
由来種
Mouse -
特異性
Recognises human Mcm2 -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to MCM2. Bacterially expressed human Mcm2.
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ポジティブ･コントロール
- Tonsil, any tissue with high proliferative index
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特記事項
Clone Number: CRCT2.1 (D1.9H5)Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
CRCT2.1 (D1.9H5) -
ミエローマ
Sp2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4171 Human
- Omim: 116945 Human
- SwissProt: P49736 Human
- Unigene: 477481 Human
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別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
画像
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Western blot - Anti-MCM2 antibody [CRCT2.1 (D1.9H5)] (ab6153)Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
参考文献 (10)
ab6153 は 10 報の論文で使用されています。
- Wienert B et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun 11:2109 (2020). PubMed: 32355159
- Mishra S et al. Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications. Cell 174:803-817.e16 (2018). PubMed: 30057114
- Scapulatempo-Neto C et al. Characterization of topoisomerase II a and minichromosome maintenance protein 2 expression in anal carcinoma. Oncol Lett 13:1891-1898 (2017). IHC-P ; Human . PubMed: 28454340
- Zhang AT et al. Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124:2058-69 (2011). PubMed: 21610089
- Shtutman M et al. Function-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing. Proc Natl Acad Sci U S A 107:7377-82 (2010). WB ; Human . PubMed: 20368428
- Sarfraz S et al. Altered expression of cell cycle and apoptotic proteins in chronic hepatitis C virus infection. BMC Microbiol 8:133 (2008). IHC-P ; Human . PubMed: 18680610
- Villadsen R et al. Evidence for a stem cell hierarchy in the adult human breast. J Cell Biol 177:87-101 (2007). PubMed: 17420292
- Niklaus AL et al. Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. Hum Reprod 22:1778-88 (2007). PubMed: 17371803
- Canela N et al. Identification of an hexapeptide that binds to a surface pocket in cyclin A and inhibits the catalytic activity of the complex cyclin-dependent kinase 2-cyclin A. J Biol Chem 281:35942-53 (2006). WB ; Human . PubMed: 17001081
- Duursma A & Agami R p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation. Mol Cell Biol 25:6937-47 (2005). WB . PubMed: 16055707
