Anti-MCM2 抗体 (ab3723)
Key features and details
- Goat polyclonal to MCM2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM2 antibody
MCM2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MCM2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human MCM2 aa 1-50.
Database link: NP_0045117.2 -
ポジティブ･コントロール
- HeLa cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa). |
ターゲット情報
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機能
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. -
配列類似性
Belongs to the MCM family.
Contains 1 MCM domain. -
翻訳後修飾
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
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別名
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
画像
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Western blot - Anti-MCM2 antibody (ab3723)Sample: Hela cell RIPA extract (60
µ g).Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.
Detection: ECL.
Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL. -
Western blot - Anti-MCM2 antibody (ab3723)All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml
Lane 1 : NIH3T3 cells
Lane 2 : TCMK-1 cells
Lane 3 : 4T1 cells
Lane 4 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 120 kDaDetection:Chemiluminescence with an exposed time of 3 minutes.
参考文献 (0)
ab3723 は論文での使用が確認できていません。
