Key features and details
- Goat polyclonal to MCM2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).|
機能Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
配列類似性Belongs to the MCM family.
Contains 1 MCM domain.
翻訳後修飾Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Information by UniProt
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.
Detection: ECL.Sample: Hela cell RIPA extract (60µg). Antibody: Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection: ECL.
All lanes : Anti-MCM2 antibody (ab3723) at 0.5 µg/ml
Lane 1 : NIH3T3 cells
Lane 2 : TCMK-1 cells
Lane 3 : 4T1 cells
Lane 4 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 120 kDa
Detection:Chemiluminescence with an exposed time of 3 minutes.