Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
Loading amount
20 µg
Specification
HeLa cells and BJ human fibroblast cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: Promega AP-anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/5000
Detection
Detection method
colorimetric AP
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 140, 90 through 50 kDa
Additional data
Additional Notes
lane 1 - Vero extract
lane 2 - HeLa extract
lane 3 - fibroblast extract
PVDF membrane
extracts were lysed in sample buffer
lane 2 - HeLa extract
lane 3 - fibroblast extract
PVDF membrane
extracts were lysed in sample buffer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 03 2008