Anti-MCM10 抗体 (ab3733)
Key features and details
- Rabbit polyclonal to MCM10
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MCM10 antibody
MCM10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM10 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Orangutan
非交差種:
Xenopus laevis, Drosophila melanogaster -
免疫原
Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.
-
ポジティブ･コントロール
- HeLa cell lysate.
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
ターゲット情報
-
機能
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. -
配列類似性
Belongs to the MCM10 family. -
発生段階
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase. -
ドメイン
The zinc finger-like domain binds a zinc ion and is involved in self-association. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli. - Information by UniProt
-
参照データベース
- Entrez Gene: 55388 Human
- Entrez Gene: 70024 Mouse
- Omim: 609357 Human
- SwissProt: Q3MIR3 Human
- SwissProt: Q5T670 Human
- SwissProt: Q7L590 Human
- SwissProt: Q9H0D7 Human
- SwissProt: Q9H3P9 Human
see all -
別名
- CNA43 antibody
- DNA43 antibody
- FLJ31133 antibody
see all
画像
-
Western blot - Anti-MCM10 antibody (ab3733)
Sample: Hela cell lysate (1mg for IP).
Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.
Detection: ECL.
Sample: Hela cell lysate (1mg for IP).
Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.
Detection: ECL.
参考文献 (4)
ab3733 は 4 報の論文で使用されています。
- Ma Y et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). PubMed: 29285216
- Liao YX et al. Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells. Mol Med Rep 13:4872-8 (2016). PubMed: 27082503
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359
- Im JS et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). PubMed: 19805216
