Anti-MCM10 抗体 (ab3733)

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Western blot - Anti-MCM10 antibody (ab3733)

    Key features and details

    • Rabbit polyclonal to MCM10
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-MCM10 antibody
      MCM10 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to MCM10

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Orangutan非交差種: Xenopus laevis, Drosophila melanogaster

    • 免疫原

      Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.

    • ポジティブ･コントロール

      • HeLa cell lysate.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • バッファー

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3733の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
    特記事項
    WB
    1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

    ターゲット情報

    • 機能

      Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication.

    • 配列類似性

      Belongs to the MCM10 family.

    • 発生段階

      Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase.

    • ドメイン

      The zinc finger-like domain binds a zinc ion and is involved in self-association.

    • 翻訳後修飾

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 細胞内局在

      Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • CNA43 antibody
      • DNA43 antibody
      • FLJ31133 antibody
      • Homolog of yeast MCM10 antibody
      • HsMCM10 antibody
      • mcm10 antibody
      • MCM10 minichromosome maintenance deficient 10 antibody
      • MCM10_HUMAN antibody
      • Minichromosome maintenance 10 replication initiation factor antibody
      • Minichromosome maintenance complex component 10 antibody
      • Minichromosome maintenance protein 10 isoform 1 antibody
      • PRO2249 antibody
      • Protein MCM10 homolog antibody
      see all

    画像

    • Western blot - Anti-MCM10 antibody (ab3733)
      Western blot - Anti-MCM10 antibody (ab3733)

      Sample: Hela cell lysate (1mg for IP).

      Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

      Detection: ECL.

       

      Sample: Hela cell lysate (1mg for IP).

      Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.

      Detection: ECL.

    参考文献 (4)

    ab3733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3733 は 4 報の論文で使用されています。

    • Ma Y  et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). PubMed: 29285216
    • Liao YX  et al. Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells. Mol Med Rep 13:4872-8 (2016). PubMed: 27082503
    • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359
    • Im JS  et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). PubMed: 19805216

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    Western blot abreview for Anti-MCM10 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells and BJ human fibroblast cells)
    Loading amount
    20 µg
    Specification
    HeLa cells and BJ human fibroblast cells
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Jun 03 2008

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