Anti-MCM10 抗体 (ab3733)
Key features and details
- Rabbit polyclonal to MCM10
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MCM10 antibody
MCM10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MCM10 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Orangutan
非交差種:
Xenopus laevis, Drosophila melanogaster -
免疫原
Synthetic peptide (Human) - which represented a portion of Minichromosome Maintenance Deficient 10 encoded within exon 14.
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ポジティブ･コントロール
- HeLa cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use a concentration of 1 - 4 µg/ml. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
ターゲット情報
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機能
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. -
配列類似性
Belongs to the MCM10 family. -
発生段階
Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G(1) phase, and starts to accumulate at the onset of S phase. -
ドメイン
The zinc finger-like domain binds a zinc ion and is involved in self-association. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli. - Information by UniProt
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参照データベース
- Entrez Gene: 55388 Human
- Entrez Gene: 70024 Mouse
- Omim: 609357 Human
- SwissProt: Q3MIR3 Human
- SwissProt: Q5T670 Human
- SwissProt: Q7L590 Human
- SwissProt: Q9H0D7 Human
- SwissProt: Q9H3P9 Human
see all -
別名
- CNA43 antibody
- DNA43 antibody
- FLJ31133 antibody
see all
画像
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Western blot - Anti-MCM10 antibody (ab3733)Sample: Hela cell lysate (1mg for IP).
Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.
Detection: ECL.
Sample: Hela cell lysate (1mg for IP).
Antibody: Affinity purified Rabbit anti-MCM 10 (ab3733); 1mg for IP, 1 / 2000 dilution for WB.
Detection: ECL.
プロトコール
参考文献 (4)
ab3733 は 4 報の論文で使用されています。
- Ma Y et al. Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. Oncotarget 8:104813-104830 (2017). PubMed: 29285216
- Liao YX et al. Silencing of RTKN2 by siRNA suppresses proliferation, and induces G1 arrest and apoptosis in human bladder cancer cells. Mol Med Rep 13:4872-8 (2016). PubMed: 27082503
- Miotto B et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . PubMed: 24726359
- Im JS et al. Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. Proc Natl Acad Sci U S A 106:15628-32 (2009). PubMed: 19805216
