製品名MCF7 nuclear extract lysate
MCF7 ライゼート 製品一覧
Extracts have been quality control tested by Western blot.
保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
Concentration information loading...
MCF7 nuclear extract lysate was prepared from cell cultures of the human MCF7 breast adenocarcinoma cancer cell line. The MCF7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer
背景MCF-7 cells are a well-characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin , endothelin , GFAP, neurofilament, vimentin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.