Anti-MC4-R 抗体 (ab24233)
Key features and details
- Rabbit polyclonal to MC4-R
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MC4-R antibody
MC4-R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MC4-R -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
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特記事項
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
精製度
Protein A purified -
一次抗体 備考
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24233の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Mouse MC4-R peptide (ab78212). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein.
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| IHC-P | (1) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Mouse MC4-R peptide (ab78212). A larger band is also detected at 55 kDa. This most likely represents the glycosylated form of the protein. |
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IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. This receptor is mediated by G proteins that stimulate adenylate cyclase. -
組織特異性
Brain, placental, and gut tissues. -
関連疾患
Defects in MC4R are a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4160 Human
- Entrez Gene: 25635 Rat
- Omim: 155541 Human
- SwissProt: P32245 Human
- SwissProt: P70596 Rat
- Unigene: 532833 Human
- Unigene: 10620 Rat
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別名
- MC4 Receptor antibody
- MC4-R antibody
- MC4R antibody
see all
画像
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Western blot - Anti-MC4-R antibody (ab24233)All lanes : Anti-MC4-R antibody (ab24233)
Lane 1 : Human cerebella cortex pellet.
Lane 2 : Human hippocampus homogenate.
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Additional bands at: 55 kDa (possible glycosylated form) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC4-R antibody (ab24233)Immunoperoxidase staining of rat brain section with ab24233. Note the tadpole like staining of cortex neurons.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab24233 は 17 報の論文で使用されています。
- Yu S et al. Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats. J Neuroinflammation 18:26 (2021). PubMed: 33468172
- Huang XM et al. Mutations in MC4R facilitate the angiogenic activity in patients with orbital venous malformation. Exp Biol Med (Maywood) 245:956-963 (2020). PubMed: 32363922
- Wang K et al. Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain. Open Life Sci 15:580-587 (2020). PubMed: 33817246
- Semple E et al. Oxytocin Neurons Enable Melanocortin Regulation of Male Sexual Function in Mice. Mol Neurobiol 56:6310-6323 (2019). PubMed: 30756300
- Ma Q et al. Xiaoyaosan Ameliorates Chronic Immobilization Stress-Induced Depression-Like Behaviors and Anorexia in Rats: The Role of the Nesfatin-1-Oxytocin-Proopiomelanocortin Neural Pathway in the Hypothalamus. Front Psychiatry 10:910 (2019). PubMed: 31920757