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MBD1 peptide (ab4918)

Submit a review Q&A (5)References (1)

Key features and details

  • Suitable for: Blocking

製品の詳細

特性

Our Abpromise guarantee covers the use of ab4918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking

  • 製品の状態

    Liquid

  • 備考


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and human MBD 1, catalog ab2846. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

関連情報

  • 別名

    • CXXC 3
    • CXXC-type zinc finger protein 3
    • CXXC3
    • MBD 1
    • Mbd1
    • MBD1_HUMAN
    • MECP1 COMPLEX
    • Methyl CpG binding domain protein 1
    • Methyl CpG binding domain protein 1 isoform PCM1
    • Methyl CpG binding protein MBD1
    • Methyl CpG binding protein splice variant 1
    • Methyl CpG binding protein splice variant 2
    • Methyl CpG binding protein splice variant 3
    • Methyl CpG binding protein splice variant 4
    • Methyl-CpG-binding domain protein 1
    • Methyl-CpG-binding protein MBD1
    • PCM 1
    • PCM1
    • Protein containing methyl-CpG-binding domain 1
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription
    • RFT
    • The regulator of fibroblast growth factor 2(FGF 2) transcription
    see all

  • 機能

    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.

  • ドメイン

    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

  • 翻訳後修飾

    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab4918 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4918 は 1 報の論文で使用されています。

  • Cerf A  et al. Single DNA Molecule Patterning for High-Throughput Epigenetic Mapping. Anal Chem : (2011). PubMed: 21981444

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Question

Thank you for your reply.
I have another question. As I see in thewebsite, the MBD1 peptide can not bind to methylated DNA in the FAQ page. Then, do you have the MBD1 protein that can bind to methylated dsDNA ? And it would be better if the biotinylated form is also available.
Looking forward to your reply.
Best egards,

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Answer

Thank you for contacting us.

The binding with methylated DNA is related to the peptide ab4918.Please chek this is not a prtoein it is a blocking peptide whihc was used to producty antibody ab2846. This is not a fucntional peptide.

The other proteins are full length (ab152513) is full length protein whih chas been tested in WB, SDS-PAGE and ELISA. These applicaiotn doe snot involve any kind of binding with DNA so I am sorry we will not be able to comment on the binding specificity of this protein.

We unfortunaltely do not have Biotin conjugated proteins avialable.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to know the component of storage solution for MBD1 peptide (ab4918).

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Answer

The peptide is dissolved in water without any salts. For storage in your lab, just aliquot.

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Question

Dear technical,

ab126003,ab4918
He knows those proteins are not guaranteed for ELISA but needs for his test with antibodies. I searched ab89534 and ab71007 for MBD1. Please let me know which one is better choice and give me information about MBD4 antibody. If you recommend one of antibody from your catalog, I'll introduce it with discount code.

Best regards

Read More
Answer

Thank you for your inquiry.

MBD1 :

I would like to point out that ab4918 is 13 amino acid synthetic peptide whose sequences are derived from human MBD1 protein, C-R(593)SKDLKKPGARK(605) (amino to carboxyl terminus). This peptide can be used with studies using ab2846, but not with other antibodies since it is only a very short peptide.

ab71007 and ab89534 (both anti MBD1 antibodies cannot be used with ab4918. They will not bind this specific sequence.

Both these antibodies will work with a full length protein. Unfortunately, we do not currently offer this in our catalog.

MBD4 :

ab126003 is a recombinant fragment of Human MBD4 (amino acids 68-300) with a His-DHFR tag.
Please be aware thatab84754 is not compatible with ab126003.

I am happy to confirm that ab124460 (https://www.abcam.com/MBD4MED1-antibody-ab124460.html (or use the following: https://www.abcam.com/MBD4MED1-antibody-ab124460.html).)will bind ab126003 as it is the immunogen sequence.

Butab124460 is not tested for ELISA. I could offer your customer to take part in the testing discount program for this antibody in ELISA.

I am sorry that I did not have a 100% positive answer for your customer and hope this information is nevertheless helpful.

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Question

Dear Jeremy, Thank you for your reply. I think so too. Please see: http://pubs.acs.org/doi/pdfplus/10.1021/ac202506j

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Answer

Thanks for sending the link. Unfortunately we don't have a subscription so I was not able to open the paper. Do you have a PDF version of the paper?

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Question

Dear Abcam I have a question about your MBD1 peptide is ab4918. Can the peptide bind to 5-methyl cytosine on DNA?

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Answer

Thank you for your enquiry. ab4918 is not a functional peptide of any kind. It is just a short peptide used as the immunogen to produce Anti-MBD1 antibody - ChIP Grade (ab2846). So it will not bind 5-methyl cytosine on DNA. I hope this is helpful. Please contact me again if you have any further questions.

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