Anti-MBD1 抗体 (ab2846)

Reviews (1)Q&A (1)References (7)
Western blot - Anti-MBD1 antibody (ab2846)

    Key features and details

    • Rabbit polyclonal to MBD1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-MBD1 antibody
      MBD1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to MBD1

    • 由来種

      Rabbit

    • 特異性

      This antibody detects human methyl CpG binding domain protein 1 (MBD1) recombinant protein.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human MBD1 aa 593-605.
      Sequence:

      RSKDLKKPGARKQ


      (Peptide available as ab4918)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab2846 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with MBD1 peptide (ab4918).

    ターゲット情報

    • 機能

      Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

    • 組織特異性

      Widely expressed.

    • 配列類似性

      Contains 3 CXXC-type zinc fingers.
      Contains 1 MBD (methyl-CpG-binding) domain.

    • ドメイン

      The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
      The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
      The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.

    • 翻訳後修飾

      Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 細胞内局在

      Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CXXC 3 antibody
        • CXXC-type zinc finger protein 3 antibody
        • CXXC3 antibody
        • MBD 1 antibody
        • Mbd1 antibody
        • MBD1_HUMAN antibody
        • MECP1 COMPLEX antibody
        • Methyl CpG binding domain protein 1 antibody
        • Methyl CpG binding domain protein 1 isoform PCM1 antibody
        • Methyl CpG binding protein MBD1 antibody
        • Methyl CpG binding protein splice variant 1 antibody
        • Methyl CpG binding protein splice variant 2 antibody
        • Methyl CpG binding protein splice variant 3 antibody
        • Methyl CpG binding protein splice variant 4 antibody
        • Methyl-CpG-binding domain protein 1 antibody
        • Methyl-CpG-binding protein MBD1 antibody
        • PCM 1 antibody
        • PCM1 antibody
        • Protein containing methyl-CpG-binding domain 1 antibody
        • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
        • RFT antibody
        • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
        see all

      画像

      • Western blot - Anti-MBD1 antibody (ab2846)
        Western blot - Anti-MBD1 antibody (ab2846)

        Western blot showing approx 83 kD band, representing recombinant human MBD 1.

      参考文献 (7)

      ab2846 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2846 は 7 報の論文で使用されています。

      • K N H  et al. Sex-Based Mhrt Methylation Chromatinizes MeCP2 in the Heart. iScience 17:288-301 (2019). PubMed: 31323475
      • Takeda Y  et al. Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II. J Am Heart Assoc 7:N/A (2018). PubMed: 29739797
      • Arosh JA  et al. Selective inhibition of prostaglandin E2 receptors EP2 and EP4 modulates DNA methylation and histone modification machinery proteins in human endometriotic cells. Mol Cell Endocrinol 409:51-8 (2015). PubMed: 25843056
      • Laget S  et al. MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress. Epigenetics 9:546-56 (2014). PubMed: 24434851
      • Katryniok C  et al. Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity. Biochim Biophys Acta 1801:49-57 (2010). WB, ChIP ; Human . PubMed: 19781662
      • Müller I  et al. Promoter- and cell-specific epigenetic regulation of CD44, Cyclin D2, GLIPR1 and PTEN by methyl-CpG binding proteins and histone modifications. BMC Cancer 10:297 (2010). WB ; Mouse . PubMed: 20565761
      • Auriol E  et al. Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus. Nucleic Acids Res 33:4243-54 (2005). ChIP ; Human . PubMed: 16052033

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      ChIP abreview for Anti-MBD1 antibody - ChIP Grade

       Good
      Abreviews
      Application
      ChIP
      Sample
      Human Cell lysate - nuclear (Breast Cancer Cell Line MCF-7)
      Specification
      Breast Cancer Cell Line MCF-7
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 0 second(s)
      Specification of the cross-linking agent: Formaldehyde
      Detection step
      Real-time PCR
      Positive control
      Amplification of sonicated genomic DNA (gDNA) with gene-specific primers prior immunoprecipitation served as positive control.
      Negative control
      DNA immunoprecipitated with the antibody IgG served as negative control
      Read More

      Dr. Heidi Schwarzenbach

      Verified customer

      投稿 May 01 2007

      Question

      I have a question about two antibodies against MBD1 you offer. The catalog number for the first one is ab3753 and the other is ab2846 both detect the same protein with all his isoforms, but the molecular weight is very different. The first one ab3753 shows a band at 60kDa [your website says: Synthetic peptide: KQEPPDPEEDKEENKDDSAS (Human).This represents the C-terminal amino acids of all human MBD 1 isoforms. WB: Use at a dilution of 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 57.6 kDa)]. and the other ab2846 at 83kDa [your website: Synthetic peptide: C-RSKDLKKPGARKQ, corresponding to amino acids 593-605 of Human MBD 1 WB: Use at a concentration of 2 - 5 ?g/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa)]. I hope you are able to explain this.

      Read More
      Answer

      Thank you for your enquiry and for your patience. I have been looking into the issue that you have raised. Both ab3753 and ab2846 are originated outside Abcam and by two different sources. The originator of ab2846 said that all evidence that they have shows this antibody detecting a band at ~83 kDa. The SwissProt database link does say the molecular weight is 57.6 kDa. Ab2846 was only tested on recombinant MBD1 protein which may be the reason for the different molecular weights in the antibodies. I'm still investigating this issue and will let you of any additional information that I obtain.

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