Anti-MAX 抗体 (ab101271)
Key features and details
- Rabbit polyclonal to MAX
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MAX antibody
MAX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MAX -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide corresponding to Human MAX aa 100-200.
Database link: P61244 -
ポジティブ･コントロール
- HeLa and 293T whole cell lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human ovarian tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 18 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 18 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
組織特異性
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
関連疾患
Pheochromocytoma -
配列類似性
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
翻訳後修飾
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
細胞内局在
Nucleus. Cell projection, dendrite. - Information by UniProt
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参照データベース
- Entrez Gene: 4149 Human
- Entrez Gene: 17187 Mouse
- Omim: 154950 Human
- SwissProt: P52162 Chicken
- SwissProt: P61244 Human
- SwissProt: P28574 Mouse
- Unigene: 285354 Human
- Unigene: 268548 Mouse
see all -
別名
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAX antibody (ab101271)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAX with ab101271 at 1/1000 (1µg/ml).
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Western blot - Anti-MAX antibody (ab101271)All lanes : Anti-MAX antibody (ab101271) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-MAX antibody (ab101271)Detection of MAX in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101271 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents Immunoprecipitates from control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab101271 は 4 報の論文で使用されています。
- Yamashita T et al. Decreased MYC-associated factor X (MAX) expression is a new potential biomarker for adverse prognosis in anaplastic large cell lymphoma. Sci Rep 10:10391 (2020). PubMed: 32587329
- Chang X et al. A Novel Phenotype of Germline Pathogenic Variants in MAX: Concurrence of Pheochromocytoma and Ganglioneuroma in a Chinese Family and Literature Review. Front Endocrinol (Lausanne) 11:558 (2020). PubMed: 32973681
- Nicolson NG et al. Transcription Factor Profiling Identifies Spatially Heterogenous Mediators of Follicular Thyroid Cancer Invasion. Endocr Pathol 31:367-376 (2020). PubMed: 33063251
- Malchenko S et al. Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. PLoS One 12:e0173106 (2017). IHC-P ; Human . PubMed: 28249000
