製品名Anti-MAT1A antibody [EPR7938] - BSA and Azide free
MAT1A 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7938] to MAT1A - BSA and Azide free
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human MAT1A aa 50-150. The exact sequence is proprietary.
- IP: 293T cells.
Ab248332 is the carrier-free version of ab129176. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248332 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 1.22 x 10 -11 M Learn more about KD
Concentration information loading...
精製度Protein A purified
Our Abpromise guarantee covers the use of ab248332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
関連性MAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. Mutations in this gene are associated with methionine adenosyltransferase deficiency.
- AdoMet antibody
- AdoMet synthetase 1 antibody
- AI046368 antibody
ab129176 (Purified) at 1:40 dilution (2 µg) immunoprecipitating MAT1A in 293T whole cell lysate.
Lane 1 (input): 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab129176 & 293T whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129176 in 293T whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129176)
ab248332 has not yet been referenced specifically in any publications.