Anti-Mast Cell Tryptase 抗体 [EPR9522] - BSA and Azide free (ab271916)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)](https://www.abcam.co.jp/ps/products/271/ab271916/Images/ab271916-1-ab151757158877antimastcelltryptaseantibodyepr9522immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9522] to Mast Cell Tryptase - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
製品の概要
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製品名
Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free
Mast Cell Tryptase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9522] to Mast Cell Tryptase - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
Synthetic peptide corresponding to Human Mast Cell Tryptase aa 1-300.
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ポジティブ･コントロール
- Human skin, colon cancer or tonsil lysate, Human colon tissue.
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特記事項
ab271916 is the carrier-free version of ab151757. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9522 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| All applications |
Mouse
Rat
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
|
| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
ターゲット情報
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関連性
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation. -
細胞内局在
Secreted. Note: released from the secretory granules upon mast cell activation. -
参照データベース
- Entrez Gene: 7177 Human
- Entrez Gene: 100503895 Mouse
- Entrez Gene: 54271 Rat
- Omim: 191080 Human
- SwissProt: Q15661 Human
- SwissProt: Q02844 Mouse
- SwissProt: P27435 Rat
- Unigene: 405479 Human
see all -
別名
- alpha II antibody
- Lung tryptase antibody
- Mast cell alpha II tryptase antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)
Immunohistochemical analysis of paraffin embedded Human tissue labeling Mast Cell Tryptase with ab151757 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151757). -
![Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)](https://www.abcam.co.jp/ps/products/271/ab271916/Images/ab271916-2-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Tryptase antibody [EPR9522] - BSA and Azide free (ab271916)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab271916 は論文での使用が確認できていません。
