Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon)
Specification
colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization
Yes - Triton-X
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 24°C
Other product details
Dilution
1/250
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 5°C
Secondary antibody
Name
Abcam antibody:Goat anti-Mouse IgG H&L (FITC) secondary antibody
Dilution
1/2000
Additional data
Additional Notes
FFPE tissue cut at one micron, counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Tom Donndelinger
Verified customer
投稿 Nov 29 2007