Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow derived mast cells)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Bone marrow derived mast cells
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% BSA in 1x PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Thermo Fisher donkey anti-mouse Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Staining was not as granular as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Nicole Corder
Verified customer
投稿 Jul 02 2018