Anti-Mast Cell Chymase 抗体 [CC1] (ab2377)
製品の概要
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製品名Anti-Mast Cell Chymase antibody [CC1]
Mast Cell Chymase 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [CC1] to Mast Cell Chymase
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由来種Mouse
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特異性Ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
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アプリケーション適用あり: IHC-P, IHC-Fr, Flow Cytmore details
適用なし: WB -
種交差性交差種: Rat, Human, Pig
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免疫原
Purified human skin Chymase.
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ポジティブ･コントロール
- Tonsil. IHC-P: FFPE human tonsil tissue sections.
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特記事項
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度Protein G purified
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一次抗体 備考Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
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ポリ/モノモノクローナル
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クローン名CC1
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ミエローマunknown
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アイソタイプIgG1
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab2377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| IHC-P | Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. 1/100-1/200 in an ABC method. | |
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
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組織特異性Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
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配列類似性Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在Secreted. Cytoplasmic granule. Mast cell granules.
- Information by UniProt
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参照データベース
- Entrez Gene: 1215 Human
- Entrez Gene: 25627 Rat
- Omim: 118938 Human
- SwissProt: P23946 Human
- SwissProt: P50339 Rat
- Unigene: 135626 Human
- Unigene: 10182 Rat
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別名
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.co.jp/ps/products/2/ab2377/Images/ab2377-266736-ap2189339assy49979edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.co.jp/ps/products/2/ab2377/Images/ab2377-266737-ID1076ab2377AP218933930secDATASHEETIMAGE.jpg)
Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml
Lane 1 : Recombinant Human Chymase Protein
Lane 2 :Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 secondsAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
参考文献
This product has been referenced in:
- Zheng J et al. Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS One 9:e94732 (2014). ICC ; Mouse . Read more (PubMed: 24733352) »
- Yadav A et al. Altered immunohistochemical expression of mast cell tryptase and chymase in the pathogenesis of oral submucous fibrosis and malignant transformation of the overlying epithelium. PLoS One 9:e98719 (2014). IHC-P ; Human . Read more (PubMed: 24874976) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"