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    mast-cell-chymase-antibody-cc1-ab2377.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Mast Cell Chymase 抗体 [CC1] (ab2377)

  • Datasheet
  • SDS
Reviews (3)Q&A (17)References (27)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
  • Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)

Key features and details

  • Mouse monoclonal [CC1] to Mast Cell Chymase
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Mast Cell Chymase antibody [CC1]
    Mast Cell Chymase 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [CC1] to Mast Cell Chymase
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: WB
  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat, Pig
  • 免疫原

    corresponding to Mast Cell Chymase.

  • ポジティブ・コントロール

    • Tonsil. IHC-P: FFPE human tonsil tissue sections.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    Protein G purified
  • 一次抗体 備考

    Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
  • ポリ/モノ

    モノクローナル
  • クローン名

    CC1
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Other
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2377の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能

      Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
    • 組織特異性

      Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
    • 配列類似性

      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.
    • 細胞内局在

      Secreted. Cytoplasmic granule. Mast cell granules.
    • Target information above from: UniProt accession P23946 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1215 Human
      • Entrez Gene: 25627 Rat
      • Omim: 118938 Human
      • SwissProt: P23946 Human
      • SwissProt: P50339 Rat
      • Unigene: 135626 Human
      • Unigene: 10182 Rat
      • 別名

        • Alpha-chymase antibody
        • Chymase 1 antibody
        • Chymase 1 mast cell antibody
        • chymase 1 preproprotein transcript E antibody
        • chymase 1 preproprotein transcript I antibody
        • Chymase antibody
        • Chymase, heart antibody
        • Chymase, mast cell antibody
        • CMA1 antibody
        • CMA1_HUMAN antibody
        • CYH antibody
        • CYM antibody
        • EC 3.4.21.39 antibody
        • Mast cell chymase 1 antibody
        • Mast cell protease 3 antibody
        • Mast cell protease 5 antibody
        • Mast cell protease I antibody
        • Mast cell protease III antibody
        • Mcp-5 antibody
        • MCP3P antibody
        • Mcpt5 antibody
        • MCT1 antibody
        • MGC119890 antibody
        • MGC119891 antibody
        • MMCP-5 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)

        IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

         

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

        *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

      • Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
        Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
        All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml

        Lane 1 : Recombinant Human Chymase Protein
        Lane 2 : Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)

        Lysates/proteins at 0.1 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 27 kDa
        Observed band size: 35 kDa why is the actual band size different from the predicted?


        Exposure time: 30 seconds


         

         

        Ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.

      プロトコール

      • Immunohistochemistry protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (27)

      ab2377 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2377 は 27 報の論文で使用されています。

      • Kurihara-Shimomura M  et al. Mast cell chymase promotes angiogenesis and lymphangiogenesis mediated by activation of melanoma inhibitory activity gene family members in oral squamous cell carcinoma. Int J Oncol 56:1093-1100 (2020). PubMed: 32319583
      • Butts B  et al. Reduced Left Atrial Emptying Fraction and Chymase Activation in Pathophysiology of Primary Mitral Regurgitation. JACC Basic Transl Sci 5:109-122 (2020). PubMed: 32140620
      • Atiakshin D  et al. Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol 152:253-269 (2019). PubMed: 31392409
      • Powell PC  et al. Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload. Heliyon 5:e01397 (2019). PubMed: 30997426
      • Wu D  et al. Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies. Sci Rep 9:19375 (2019). PubMed: 31852975
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 20 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Chymase antibody [CC1]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
      Permeabilization
      No
      Specification
      skin
      Blocking step
      Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 15 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Mast Cell Chymase antibody [CC1]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
      Sample
      Human Cell (Mast cell)
      Specification
      Mast cell
      Permeabilization
      No
      Fixative
      BD Biosciences fixation/permeabilization solution
      Read More

      Abcam user community

      Verified customer

      投稿 Aug 05 2014

      Question

      Thank you so much! I appreciate it.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 14 2012

      Answer

      You are very welcome.

      Please let us know if there are ever any other ways in which Abcam might help you.

      Read More

      Abcam Scientific Support

      Answered on Jun 14 2012

      Question

      We just had a lab discussion this morning, we decided to take the "Mouse monoclonal [CC1] to Mast Cell Chymase" with the cat# ab2377.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 14 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Jun 14 2012

      Question

      I'm currently looking for an antibody against mast cell chymase for use
      in the rat. I see you have the ab111239 antibody designed against human
      mast cell chymase. Would it be possible to have the immunogen sequence
      so that I can assess whether this would be suitable for use in rat
      tissue as well?
      Thanks and regards,

      Read More

      Abcam community

      Verified customer

      Asked on May 08 2012

      Answer

      Thank you for contacting us.

      The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

      We unfortunately do not have any extra immunogen information other than this. We also haven't
      tested these antibodies with rat sample so can't confirm whether these abs will be suitable. The end users have to try for cross reactivity check by themselves. If you are interested in testing, I am happy offering you a 100% Abreview discount code which will entitles you to purchase one antibody free of charge, if you buy ab111239 and submit a RAT specific Abreview.

      Full terms and conditions can be found at the following link.

      https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 08 2012

      Question

      wir hatten im März Kontakt, wegen meiner Fragen zum Chymase Antikörper von Abcam mit der Nummer: ab2377.



      Meine Immunhistofärbungen sehen gut aus. Nun stellt sich die Frage, ob man zusätzlich zur IHC mit ab2377 eine Färbung mit Sirius Red vornehmen kann?!

      Haben Sie damit Erfahrungen? Was würden Sie mir empfehlen?



      Mein Kurz-Protokoll:

      Entparaffinierung

      Demaskierung mit Citratpuffer

      Blocken mit PBS/BSA 1%

      1.AK ab2377 Ü.N.

      Waschen

      2.Ak HRP Goat Anti-Mouse

      Waschen

      DAPI Färbung/Zellkernfärbung

      Waschen

      Eindecken mit Mounting Medium



      Bei welchem Schritt würden Sie die Sirius red Färbung einbauen? Nach der DAPI Färbung? Oder sollte ich diese weglassen und die Sirius red Färbung nach dem 2.Ak beginnen?



      Würde mich sehr über eine Antwort und Hilfe von Ihnen freuen!



      Mit freundlichen Grüßen

      Read More

      Abcam community

      Verified customer

      Asked on Apr 23 2012

      Answer

      Vielen Dank für Ihre Anfrage.

      Es freut mich sehr zu hören, dass ab2377 gute Ergebnisse liefert.

      Ich persönlich habe leider keine Erfahrung mit Sirius Red. Meine Kollegen allerdings haben Sirius Red schon verwendet. Allerding nur als Einzelfärbung in aufeinanderfolgenden Schnitten, wobei die nächsten Schnitte dann die Immunofärbung enthielten.

      Vielleicht helfen Ihnen die folgenden Websites und Publikationen bezüglich der Doppel färbung weiter:

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1867025/

      http://ndt.oxfordjournals.org/content/early/2012/02/01/ndt.gfr671.abstract

      http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm

      http://stainsfile.info/StainsFile/dyes/35780.htm

      http://www.nottingham.ac.uk/pathology/protocols/epsr.html

      Es tut mir leid, dass ich Ihnen diesmal nicht wirklich weiterhelfen konnte und wünsche Ihnen viel Erfolg mit Ihren Versuchen.

      Read More

      Abcam Scientific Support

      Answered on Apr 23 2012

      Question

      noch eine Frage:

      mit einem ihrer anderen Chymase-Ak kann ich bei Abcam ein tolles Foto mit Gegenfärbung sehen.

      Welche Gegenfärbung wurde da verwendet und kann ich diese auch bei meinem Ak verwenden?

      Die Darstellung mit DAB ist nicht optimal bzw. eindeutig zu erkennen.



      Vielen Dank für Ihre Hilfe.

      Mit freundlichen Grüßen

      Read More

      Abcam community

      Verified customer

      Asked on Mar 12 2012

      Answer

      Vielen Dank für Ihre Anfrage.

      Die Abbildung von ab54176 wurde mit einer Gegenfärbung mit Eosin (alleine) gefärbt.

      Während die Abbildung von ab111239 eine Gegenfärbung mit Hematoxlin zeigt.

      Beide können Sieauch mit ab2377 einsetzen.

      Bitte lassen Sie mich wissen, falls ich Ihre Frage falschverstanden habe.

      Ich wünsche Ihnen viel Erfolg bei Ihnen Experimenten und verbleibe

      Read More

      Abcam Scientific Support

      Answered on Mar 12 2012

      Question

      will test ab2377 with dog samples

      Read More

      Abcam community

      Verified customer

      Asked on Jan 25 2012

      Answer

      Thank you for your phone call.

      DISCOUNT CODE: xxx
      Expiration date: xxx

      I am very pleased to hear you would like to accept our offer and test ab2377 with dog samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for dog samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jan 25 2012

      Question

      I have found abcam antibodies to be very good and accordingly am interested in your anti-mast cell chymase antibody (ab2377), but we use mice and rats and it has not yet been tested in these species. Would abcam be willing to provide my lab with a free sample that we can evaluate in mouse and rat hearts? In return we would be happy to provide Abcam with any images that we obtain using the antibody.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 06 2011

      Answer

      Thank you very much for your interest in ab2377. To our knowledge, ab2377 has not been tested in mouse and rat. Therefore, I can offer a discount off a future purchase if you buy ab2377 now, test it in mouse and rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab2377 in mouse and rat. I will then send a discount code. This code must be issued before purchasing ab2377 so please wait for my reply before ordering. DISCOUNT CODE: xxx Expiration date: xxx 2. Purchase ab2377 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse and rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2377 turns out to be unsuitable for mouse and rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Oct 06 2011

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mast Cell Chymase antibody [CC1]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Kidney)
      Specification
      Kidney
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      None
      Permeabilization
      Yes - 0.3% H2O2
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
      Read More

      Prof. Chung-Ming Chen

      Verified customer

      投稿 Jan 09 2009

      1-10 of 20 Abreviews or Q&A

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      For licensing inquiries, please contact partnerships@abcam.com

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      • 感染症
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      製品・タイプ別
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