Anti-MAP4K5 抗体 (ab96551)
Key features and details
- Rabbit polyclonal to MAP4K5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MAP4K5 antibody -
製品の詳細
Rabbit polyclonal to MAP4K5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 417-482 (HCPDSESRAP QILRRQSSPS CGPVAETSSI GNGDGISKLM SENTEGSAQA PQLPRKNDKR DFPKPA) of Human MAP4K5 (NP_942089).
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ポジティブ・コントロール
- MOLT4 whole cell lysate; A431 cells; SAS xenograft; 293T, H1299, HepG2 and Raji cell lines
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96551の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 95 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 95 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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関連性
MAP4K5, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress. It appears to act upstream of the JUN N-terminal pathway. MAP4K5 interacts with both SH3 domains of the adapter proteins CRK and CRKL. This cytoplasmic protein is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 11179 Human
- Entrez Gene: 11183 Human
- Omim: 604923 Human
- SwissProt: Q9NRM2 Human
- SwissProt: Q9Y4K4 Human
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別名
- EC 2.7.11.1 antibody
- KHS antibody
- Kinase homologous to SPS1/STE20 antibody
see all
画像
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Anti-MAP4K5 antibody (ab96551) at 1/5000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 95 kDa
12% SDS PAGE -
ab96551, at 1/200 dilution, staining MAP4K5 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is merged with DNA probe.
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ab96551, at 1/100 dilution, staining MAP4K5 in paraffin-embedded SAS xenograft by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96551 は論文での使用が確認できていません。