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Anti-MAP2 抗体 [HM-2] (ab11267)

Reviews (8)Q&A (7)References (133)
Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
  • Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

Key features and details

  • Mouse monoclonal [HM-2] to MAP2
  • Suitable for: ICC, WB
  • Reacts with: Rat
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-MAP2 antibody [HM-2]
    MAP2 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [HM-2] to MAP2

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC, WBmore details

  • 種交差性

    交差種: Rat
    交差が予測される動物種: Mouse, Chicken, Cow, Human, Quail

  • 免疫原

    Full length protein corresponding to Rat MAP2. Rat brain microtubule associated proteins (MAPs).

  • ポジティブ･コントロール

    • WB: Rat brain tissue lysate. ICC: B35 cells.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11267の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (2)
Use a concentration of 2 µg/ml.

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
WB (2)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.
特記事項
ICC
Use a concentration of 2 µg/ml.

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.

ターゲット情報

画像

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
    Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Rivastigmine preserves neuronal morphology and alters sAPP secretion

    At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

    MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

    (From Figure 2A of Bailey et al)

    This image was generated using the ascites version of the product.

  • Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
    Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
    Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate

    Predicted band size: 200 kDa



    This image was generated using the ascites version of the product.

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
    Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

    Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

    This image was generated using the ascites version of the product.

参考文献 (133)

ab11267 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab11267 は 133 報の論文で使用されています。

  • Grubisha MJ  et al. MAP2 is differentially phosphorylated in schizophrenia, altering its function. Mol Psychiatry N/A:N/A (2021). PubMed: 33526823
  • Varinthra P  et al. The 4-(Phenylsulfanyl) butan-2-one Improves Impaired Fear Memory Retrieval and Reduces Excessive Inflammatory Response in Triple Transgenic Alzheimer's Disease Mice. Front Aging Neurosci 13:615079 (2021). PubMed: 33613267
  • Choi GE  et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun 12:487 (2021). PubMed: 33473105
  • Cascella R  et al. The release of toxic oligomers from a-synuclein fibrils induces dysfunction in neuronal cells. Nat Commun 12:1814 (2021). PubMed: 33753734
  • Wang H  et al. Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri-mir-375 to miR-375. Cell Biosci 11:52 (2021). PubMed: 33706799
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 15 Abreviews or Q&A

Western blot abreview for Anti-MAP2 antibody [HM-2]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Read More

DR. Alexandre Magno

Verified customer

投稿 Dec 06 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody [HM-2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
Yes - 0.3% Triton-X-100 for 15 min
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Uthra Rajamani

Verified customer

投稿 Jan 22 2021

Immunocytochemistry abreview for Anti-MAP2 antibody [HM-2]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 12 2015

Western blot abreview for Anti-MAP2 antibody [HM-2]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More

Abcam user community

Verified customer

投稿 Apr 27 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More

Dr. Aleksandra Maruszak

Verified customer

投稿 Mar 31 2014

Immunocytochemistry abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Nov 02 2012

Question

We have already ordered antibodies from other company. I just asked my boss. You can refund our money on our account. Nice to contact with you and thank you very much for your help!

Read More
Answer

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Read More

Question

Yes, the image of DAKO gfap staining is the typical astrocyte, just as the image of instruction your company provided to us with ab90601 antibody. So, you can see the significant differences!

Read More
Answer

Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let me know how you would like to proceed.

Read More

Question

to whom may be concerned: our lab ordered four antibodies from your company in July. we cannot find the order list now. ab90601 sheep polyconal to GFAP. ab11267 mouse monoclonal HM-2 to MAP2 - neuronal marker and other two secondary antibodies: ab6896 Donkey polyclonal secondary antibody to sheep IgG - H&L(FITC) and ab47052 Goat polyclonal secondary antibody to Mouse IgG - H&L(AMCA). we used the GFAP to stain astrocyte of rat brain frozen sections and MAP2 to stain neuron of rat brain frozen sections. However, we found the images of GFAP staining were very similar to neurons, clearly not astrocytes. we repeat experiment at least three times and obtain the same results. last time, we borrowed GFAP antibody from other lab ( DAKO company product ) and followed the same procedures and make comparison with your company's GFAP. We obtained typical image of astrocyte by GFAP of borrowed antibody (DAKO product). However, for your company's GFAP, we obtained images similar to neurons just like before. Besides, for the MAP2 of your company, we cannot get any typical images of neurons although we followed the same procedures as doing GFAP. For every experiment, we made negative controls. Finally, we believe there must be somthing wrong with your two primary antibodies(GFAP and MAP2). It cost us more than two weeks to do such experiments which made us very frustrated. We just want to proceed our experiment quickly. so IF possible, someone can check it for us and send us the right antibody quickly so we can continue our experiments.

Read More
Answer

We spoke just a short while ago regarding these issues you were having with ab90601 and ab11267. I have processed the replacement orders as we discussed and sent you an update via email just a few minutes ago. You should receive these 2 replacement antibodies for ab90601 and ab11267 tomorrow. I am sorry for the trouble these 2 antibodies have caused and I do hope the replacements work as expected. Please feel free to follow up with me if you need anything further.

Read More

Question

I have tested ab90601 and ab11267 in IHC-Fr and both are not working as expected. ab90601 is staining neurons clearly and it should be staining astrocytes. Another antibody to the same target stained as expected on the same sections with the same protocol. Used at 1:100. With ab11267, there was no staining of the tissue, even at 1:100 dilution of primary.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab90601 and one vial of ab11267. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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1-10 of 15 Abreviews or Q&A

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