Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (E16 Mouse brain, Free-floating sections)
Specification
E16 Mouse brain, Free-floating sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBS, 0.2%Triton X, donkey serum
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
IHC free-floating sections (not paraffin-embedded). Avidin-biotin complex amplification and Ni-DAB colour developing. In image below showing neocortex: bottom is dorsal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Randal Moldrich
Verified customer
投稿 Apr 22 2008