Application
Western blot
Sample
Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount
10 µg
Specification
mouse brain - post nuclear supernatant
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
(agent) for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/10000
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 0,1% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase rabbit anti chicken-IgY antibody G135A
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 70, 280, 280 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 06 2012