Anti-Mannose Receptor 抗体 (ab64693)
製品の概要
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製品名Anti-Mannose Receptor antibody
Mannose Receptor 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Mannose Receptor
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由来種Rabbit
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特異性
We obtained a weaker signal with mouse samples than with human samples in Western blot when the same amount of protein was used.
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アプリケーション適用あり: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.
Immunogen の所有権に関して (Peptide available as ab66694.) -
ポジティブ･コントロール
- This antibody gave a positive signal with Rat Liver Tissue Lysate and in FFPE human lung tissue sections.
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特記事項
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Alternative Products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab64693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We do not recommend methanol fixation with this antibody |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 166 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use a concentration of 1 µg/ml. Permeabilise with Tween (0.1%) |
ターゲット情報
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機能Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
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配列類似性Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 4360 Human
- Entrez Gene: 17533 Mouse
- Entrez Gene: 291327 Rat
- Omim: 153618 Human
- SwissProt: P22897 Human
- SwissProt: Q61830 Mouse
- Unigene: 75182 Human
- Unigene: 2019 Mouse
see all -
別名
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-Mannose Receptor antibody (ab64693)Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)ICC/IF image of ab64693 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64693, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, MCF-7 cells at 5µg/ml. -
Western blot - Anti-Mannose Receptor antibody (ab64693)All lanes : ab64693 at 10 µg
Lane 1 : Liver (Rat) Tissue Lysate at 10 µg
Lane 2 : Lung (Human) tissue lysate at 10 µg
Lane 3 : Lung (Rat) tissue lysate at 10 µg
Lane 4 : Lung (Mouse) tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)
Exposure time: 4 minutes -
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody (ab64693)This image is courtesy of an anonymous Abreviewab64693 staining Mannose Receptor in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.075% H2O2 for 10 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1x PBS + 10% heat inactivated FCS) for 1 hour. An undiluted HRP-conjugated Rabbit anti-rabbit IgG polyclonal was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Stafeev I et al. Low proliferative potential of adipose-derived stromal cells associates with hypertrophy and inflammation in subcutaneous and omental adipose tissue of patients with type 2 diabetes mellitus. J Diabetes Complications 33:148-159 (2019). Read more (PubMed: 30482492) »
- Fusser M et al. Cabazitaxel-loaded Poly(2-ethylbutyl cyanoacrylate) nanoparticles improve treatment efficacy in a patient derived breast cancer xenograft. J Control Release 293:183-192 (2019). Read more (PubMed: 30529259) »
