製品の概要

  • 製品名
    Anti-Mannose Receptor antibody
    Mannose Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mannose Receptor
  • 由来種
    Rabbit
  • 特異性

    We obtained a weaker signal with mouse samples than with human samples in Western blot when the same amount of protein was used.  

  • アプリケーション
    適用あり: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.

    Immunogen の所有権に関して (Peptide available as ab66694.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal with Rat Liver Tissue Lysate and in FFPE human lung tissue sections.
  • 特記事項

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 166 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

ターゲット情報

  • 機能
    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • 配列類似性
    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA541I19.1 antibody
    • C type lectin domain family 13 member D antibody
    • C-type lectin domain family 13 member D antibody
    • CD 206 antibody
    • CD206 antibody
    • CD206 antigen antibody
    • CLEC13D antibody
    • CLEC13DL antibody
    • Macrophage mannose receptor 1 antibody
    • Macrophage mannose receptor 1 like protein 1 antibody
    • Macrophage mannose receptor antibody
    • Mannose receptor C type 1 antibody
    • Mannose receptor C type 1 like 1 antibody
    • MMR antibody
    • MRC 1 antibody
    • MRC1 antibody
    • MRC1_HUMAN antibody
    • MRC1L1 antibody
    • OTTHUMP00000045206 antibody
    see all

画像

  • IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 166 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab64693 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64693, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, MCF-7 cells at 5µg/ml.
  • All lanes : ab64693 at 10 µg

    Lane 1 : Liver (Rat) Tissue Lysate at 10 µg
    Lane 2 : Lung (Human) tissue lysate at 10 µg
    Lane 3 : Lung (Rat) tissue lysate at 10 µg
    Lane 4 : Lung (Mouse) tissue lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?
    Additional bands at: 41 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)


    Exposure time: 4 minutes
  • ab64693 staining Mannose Receptor in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.075% H2O2 for 10 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1x PBS + 10% heat inactivated FCS) for 1 hour. An undiluted HRP-conjugated Rabbit anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Stafeev I  et al. Low proliferative potential of adipose-derived stromal cells associates with hypertrophy and inflammation in subcutaneous and omental adipose tissue of patients with type 2 diabetes mellitus. J Diabetes Complications 33:148-159 (2019). Read more (PubMed: 30482492) »
  • Fusser M  et al. Cabazitaxel-loaded Poly(2-ethylbutyl cyanoacrylate) nanoparticles improve treatment efficacy in a patient derived breast cancer xenograft. J Control Release 293:183-192 (2019). Read more (PubMed: 30529259) »
See all 121 Publications for this product

レビューと Q&A

1-10 of 32 Abreviews or Q&A

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab64693.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Arm)
Permeabilization
No
Specification
Arm
Blocking step
DAKO serum free blocking as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
N/a

Miss. Crystal Jones

Verified customer

投稿 Nov 02 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Nov 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Oct 11 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Oct 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lymph Node, Spleen, Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 4
Permeabilization
No
Specification
Lymph Node, Spleen, Liver
Blocking step
Ultratek HRP (anti-polyvalent), ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Sara Belluco

Verified customer

投稿 May 23 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse LLC tumor and spleen)
Permeabilization
No
Specification
Mouse LLC tumor and spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Acetone

Abcam user community

Verified customer

投稿 Jan 29 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Dec 28 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Acomys (spiny mouse) Tissue sections (spleen)
Specification
spleen
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Crystal Jones

Verified customer

投稿 Nov 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate/Ventana RiboCC
Permeabilization
No
Specification
Lung
Fixative
Paraformaldehyde

Dr. Li Li

Verified customer

投稿 May 16 2017

1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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