Anti-Mannose Receptor 抗体 [15-2] (ab8918)
Key features and details
- Mouse monoclonal [15-2] to Mannose Receptor
- Suitable for: IHC-Fr
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Mannose Receptor antibody [15-2]
Mannose Receptor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [15-2] to Mannose Receptor -
由来種
Mouse -
アプリケーション
適用あり: IHC-Frmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Purified human mannose receptor from human placental tissue.
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エピトープ
Stains extracellular domain of Mannose Receptor. -
ポジティブ･コントロール
- Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
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特記事項
The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
0.2 µm filtered antibody solution -
一次抗体 備考
The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. -
ポリ/モノ
モノクローナル -
クローン名
15-2 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab8918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | 1/50. |
ターゲット情報
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機能
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. -
配列類似性
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4360 Human
- Omim: 153618 Human
- SwissProt: P22897 Human
- Unigene: 75182 Human
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別名
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
画像
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![Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)](https://www.abcam.co.jp/ps/products/8/ab8918/Images/ab8918-282303-anti-mannose-receptor-antibody-15-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)Frozen sections of human tonsil. ab8918 was used in a dilution of 1/25 and shows staining of endothelia of lymph vessels.
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![Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)](https://www.abcam.co.jp/ps/products/8/ab8918/Images/ab8918-15881-anti-mannose-receptor-antibody-15-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is a courtesy of Pat Bellab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)](https://www.abcam.co.jp/ps/products/8/ab8918/Images/ab8918-15879-anti-mannose-receptor-antibody-15-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.
Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody. -
![Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)](https://www.abcam.co.jp/ps/products/8/ab8918/Images/ab8918-233572-anti-mannose-receptor-antibody-15-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is courtesy of an Abreview submitted by Aimée-Lys Rusesabaginaab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
プロトコール
参考文献 (46)
ab8918 は 46 報の論文で使用されています。
- Ni H et al. Exosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury. Front Neurosci 13:14 (2019). PubMed: 30733666
- Gan J et al. Accelerated wound healing in diabetes by reprogramming the macrophages with particle-induced clustering of the mannose receptors. Biomaterials 219:119340 (2019). PubMed: 31357007
- Park HR et al. Signaling pathway and structural features of macrophage-activating pectic polysaccharide from Korean citrus, Cheongkyool peels. Int J Biol Macromol 137:657-665 (2019). PubMed: 31276719
- Chrobok NL et al. Tissue Transglutaminase Appears in Monocytes and Macrophages but Not in Lymphocytes in White Matter Multiple Sclerosis Lesions. J Neuropathol Exp Neurol 78:492-500 (2019). PubMed: 31058279
- Fan W et al. Identification of CD206 as a potential biomarker of cancer stem-like cells and therapeutic agent in liver cancer. Oncol Lett 18:3218-3226 (2019). PubMed: 31452799
