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  1. Link

    mannose-receptor-antibody-15-2-ab8918.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Mannose Receptor 抗体 [15-2] (ab8918)

  • Datasheet
  • SDS
Reviews (11)Q&A (12)References (63)

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Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
  • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
  • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
  • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)

Key features and details

  • Mouse monoclonal [15-2] to Mannose Receptor
  • Suitable for: IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC PE/Cy7® PerCP/Cy5.5®

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Mannose Receptor antibody [15-2]
    Mannose Receptor 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [15-2] to Mannose Receptor
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-Frmore details
    適用なし: IHC-P
  • 種交差性

    交差種: Human
  • 免疫原

    Purified human mannose receptor from human placental tissue.

  • エピトープ

    Stains extracellular domain of Mannose Receptor.
  • ポジティブ・コントロール

    • Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
  • 特記事項

    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • 特記事項(精製)

    0.2 µm filtered antibody solution
  • 一次抗体 備考

    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • ポリ/モノ

    モノクローナル
  • クローン名

    15-2
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Mannose Receptor protein (ab152538)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8918の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (6)
1/50.
特記事項
IHC-Fr
1/50.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • 配列類似性

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P22897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 4360 Human
    • Omim: 153618 Human
    • SwissProt: P22897 Human
    • Unigene: 75182 Human
    • 別名

      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
      Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)

      Frozen sections of human tonsil. ab8918 was used in a dilution of 1/25 and shows staining of endothelia of lymph vessels.

    • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
      Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is a courtesy of Pat Bell

      ab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
      Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is courtesy of an anonymous Abreview

      Immunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.

      Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)
      Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody [15-2] (ab8918)This image is courtesy of an Abreview submitted by Aimée-Lys Rusesabagina

      ab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (63)

    ab8918 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab8918 は 63 報の論文で使用されています。

    • Wang F  et al. Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis. Stem Cell Res Ther 12:288 (2021). PubMed: 33985585
    • Hu CF  et al. K48-Linked Ubiquitination Contributes to Nicotine-Augmented Bone Marrow-Derived Dendritic-Cell-Mediated Adaptive Immunity. Vaccines (Basel) 9:N/A (2021). PubMed: 33808531
    • Yuanyuan L  et al. Myeloid NEMO deficiency promotes tumor immunosuppression partly via MCP1-CCR2 axis. Exp Cell Res 399:112467 (2021). PubMed: 33428904
    • Han GH  et al. Transplantation of tauroursodeoxycholic acid-inducing M2-phenotype macrophages promotes an anti-neuroinflammatory effect and functional recovery after spinal cord injury in rats. Cell Prolif 54:e13050 (2021). PubMed: 33960559
    • Wang SW  et al. Nobiletin Alleviates Non-alcoholic Steatohepatitis in MCD-Induced Mice by Regulating Macrophage Polarization. Front Physiol 12:687744 (2021). PubMed: 34093242
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 23 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Mannose Receptor antibody [15-2]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Embryonic Kidney)
    Specification
    Embryonic Kidney
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 14 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Sample
    Human Tissue sections (inflamed human colon)
    Specification
    inflamed human colon
    Permeabilization
    No
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 01 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Acetone
    Read More

    Ms. Aimée-Lys Rusesabagina

    Verified customer

    投稿 Sep 12 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Breast tumor)
    Specification
    Breast tumor
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    DR. Gajendra Katara

    Verified customer

    投稿 Sep 05 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose Receptor antibody [15-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Cell (Peritoneal macrophages)
    Specification
    Peritoneal macrophages
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2014

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Mukesh Jaiswal

    Verified customer

    投稿 Nov 12 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Dr. Mukesh Jaiswal

    Verified customer

    投稿 Sep 21 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [15-2]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (placenta)
    Specification
    placenta
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Dr. Varkha Agrawal

    Verified customer

    投稿 Sep 18 2012

    Question

    Inquiry: Dear Sir, I want to order the ab8918 or ab8919 antibody. I wanted to know that if these can be used for flow then whether cells should be permeabilised or not before staining. If then how to permeabilise the required cells. Also is the mannose receptor abbreviated as cd206. Please reply quickly so that I can order them.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 26 2012

    Answer

    Thank you for contacting us.

    Both of these product are tested and guaranteed to work in Flow Cytometry. These are specific against extracellular domain so the permeabilization step is optional; the antibodies would be able to stain the cells without permeabilization.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 26 2012

    Question

    Attached document will give you an idea about my experimental setup.
    Briefly,the wells of ELISA plate will be coated with a polyclonal Ab
    (ab64693). A mixture of proteins will be added containing the mannose
    receptor. Non-specific proteins will be washed off. Another Mannose
    Receptor Ab(monoclonal? ab8918) will be added to confirm the
    specificity of protein binding to well.

    I need to know the epitopes of chosen Abs, whether they are
    overlappping. Also, whether the polyclonal Ab will interfere with the
    receptor binding site, as in future experiments I shall be using the
    polyclonal Ab to isolate MR from mixture of proteins in an ELISA
    format.

    Another capture Ab (ab117644) as been suggested specific for Sandwich
    ELISA. Would that be a more suitable choice compared to the polyclonal
    Ab.

    Thanks

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank you very much for your interest in our antibodies.

    To our knowledge, ab8918 and ab64693have not been tested in ELISA and also not in sandwichELISA.

    The epitope of ab8918 was not mapped and the same is true for ab117644.

    I would recommend to use ab117644 as capture antibody since it is tested for ELISA.

    Therefore, I can offer a discount off a future purchase if you buy ab8918or ab64693 now, test it inELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of the tested antibody off a future order.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and testab8918or ab64693 in ELISA. I will then send a discount code. This code must be issued before purchasing ab8918or ab64693 so please wait for my reply before ordering.

    2. Purchase ab8918or ab64693 either by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8918or ab64693 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    1-10 of 23 Abreviews or Q&A

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