Anti-Mannan Binding Lectin/MBL 抗体 [EPR18381-156] (ab190834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18381-156] to Mannan Binding Lectin/MBL
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156]
Mannan Binding Lectin/MBL 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18381-156] to Mannan Binding Lectin/MBL -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse liver and kidney lysates; Mouse serum; Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein. IHC-P: Mouse and human liver tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18381-156 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190834の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
This antibody is not suitable for human species in WB application due to non-specific staining and high background in human cell and tissue lysates. |
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IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). This antibody is not suitable for human species in WB application due to non-specific staining and high background in human cell and tissue lysates. |
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
組織特異性
Plasma protein produced mainly in the liver. -
関連疾患
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
配列類似性
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4153 Human
- Entrez Gene: 17195 Mouse
- Omim: 154545 Human
- SwissProt: P11226 Human
- SwissProt: P41317 Mouse
- Unigene: 499674 Human
- Unigene: 30045 Mouse
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別名
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
画像
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Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution + Mouse Mannan Binding Lectin/MBL (aa19-244) recombinant protein at 0.005 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 5 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-156] (ab190834) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse serum
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDaExposure time : Lane 1: 3 seconds; Lane 2: 3 minutes; Lane 3 1 second.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse liver is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Mannan Binding Lectin/MBL with ab190834 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)(ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab190834 は論文での使用が確認できていません。