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Anti-Mannan Binding Lectin/MBL 抗体 [3B6] (ab23457)

Reviews (2)Q&A (3)References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

Key features and details

  • Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Mannan Binding Lectin/MBL antibody [3B6]
    Mannan Binding Lectin/MBL 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3B6] to Mannan Binding Lectin/MBL

  • 由来種

    Mouse

  • 特異性

    ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state.

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Mannan Binding Lectin/MBL. Human MBL purified from Human donor plasma.

  • エピトープ

    The epitope is on the head-neck region of the MBL protein chain.

  • ポジティブ･コントロール

    • Human liver, Human cancerous liver and Human brain tissues.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    3B6

  • ミエローマ

    x63-Ag8.653

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23457の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
1/250.
特記事項
IHC-P
1/250.

ターゲット情報

  • 機能

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

  • 組織特異性

    Plasma protein produced mainly in the liver.

  • 関連疾患

    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.

  • 配列類似性

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • COLEC 1 antibody
      • COLEC1 antibody
      • Collectin-1 antibody
      • HSMBPC antibody
      • Lectin, mannose-binding, soluble, 2 antibody
      • Mannan binding lectin antibody
      • Mannan binding protein antibody
      • Mannan-binding protein antibody
      • Mannose binding lectin (protein C) 2 soluble antibody
      • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
      • Mannose binding lectin (protein C) 2, soluble antibody
      • Mannose binding lectin 2 soluble antibody
      • Mannose binding lectin 2, soluble (opsonic defect) antibody
      • Mannose binding lectin antibody
      • Mannose binding lectin protein C2 soluble opsonic defect antibody
      • Mannose binding protein antibody
      • Mannose binding protein C antibody
      • Mannose binding protein C precursor antibody
      • Mannose binding protein, serum antibody
      • Mannose-binding lectin antibody
      • Mannose-binding protein C antibody
      • MBL 2 antibody
      • MBL antibody
      • MBL2 antibody
      • MBL2_HUMAN antibody
      • MBL2D antibody
      • MBP 1 antibody
      • MBP antibody
      • MBP C antibody
      • MBP-C antibody
      • MBP1 antibody
      • MBPB antibody
      • MBPC antibody
      • MBPD antibody
      • MGC116832 antibody
      • MGC116833 antibody
      • Opsonic defect antibody
      • protein C antibody
      • Soluble mannose binding lectin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      ab23457 at 1 mg/ml staining human liver by IHC-P
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

      ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [3B6] (ab23457)

      ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.

    参考文献 (14)

    ab23457 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab23457 は 14 報の論文で使用されています。

    • Haddad G  et al. Altered glycosylation of IgG4 promotes lectin complement pathway activation in anti-PLA2R1-associated membranous nephropathy. J Clin Invest 131:N/A (2021). PubMed: 33351779
    • Yadav AK  et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . PubMed: 25303045
    • Debiec H  et al. Recurrent membranous nephropathy in an allograft caused by IgG3? targeting the PLA2 receptor. J Am Soc Nephrol 23:1949-54 (2012). PubMed: 23123401
    • Aoyagi Y  et al. L-Ficolin/mannose-binding lectin-associated serine protease complexes bind to group B streptococci primarily through N-acetylneuraminic acid of capsular polysaccharide and activate the complement pathway. Infect Immun 76:179-88 (2008). ELISA ; Human . PubMed: 17938215
    • Christensen T  et al. Gene-environment interactions in multiple sclerosis: innate and adaptive immune responses to human endogenous retrovirus and herpesvirus antigens and the lectin complement activation pathway. J Neuroimmunol 183:175-88 (2007). PubMed: 17113160
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannan Binding Lectin/MBL antibody [3B6]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 22 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannan Binding Lectin/MBL antibody [3B6]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated
    Specification
    brain tissue
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Formaldehyde
    Read More

    Vlady Pavlova

    Verified customer

    投稿 Aug 15 2006

    Question

    The placental proteins were harvested using the lysis buffer containing 8M Urea and 2%CHAPS supplemented with protein inhibitors. Protein samples were prepared using Laemalli buffer with beta-ME and DTT, boiled for five minutes. The proteins were resolved on 10%SDS-PAGE and in reducing conditions. The membrane was blocked in 1% non fat dry milk in TBS over night at 4 degree. The primary antibody incubation was 1:30hr to 2:00 hr at room temperature. The membrane was washed for 3-4 times (10 min/wash) using TBST (Triton X-100, 0.02%). Secondary antibody was Goat polyclonal anti mouse IgG-HRP (1:1000 dilution), incubation time 1:00 hr at room temperature. The antibody signals were detected using ECL method.

    Read More
    Answer

    Thank you for this additional information. A few suggestions just to follow a protocol a little more similar to how we have tested. We generally recommend blocking in 5% milk in TBST and incubating the primary antibody overnight at 4 degrees. You may also wish to try changing your lysis buffer to RIPA if possible.



    At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund within 6 months of purchase.

    The Abcam Abpromise® guarantee:
    - 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
    - We guarantee our products work in the tested species and applications stated on the datasheet.
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    Please note these conditions to our Abpromise®:
    - Protocol information must be provided in order for the claim to be validated.
    - Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
    - “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
    - Fast Track antibodies are covered for ELISA against the immunizing peptide only.

    I hope this information is helpful, but please do not hesitate to contact us with further questions.

    Read More

    Question

    My lab has purchased one of your Mabs against mannose binding protein/lectin (MBL)(ab23457). Are there tissue staining results from epidermis available for any of your anti-MBL Mabs? Further do you sell human MBL? Do you know of a source for human MBL?

    Read More
    Answer

    Thank you for your enquiry. The antibody has been tested in IHC in human liver and brain sections, but we do not have those images unfortunately. The reference Holmskov UL Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59 (2000) has used the antibody in immunohistochemistry and should have some images with this antibody. We currently do not sell recombinant human MBL and unfortunately I do not know of a source of such a product. You may be able to find this information from the website www.biocompare.com or the supplier Sigma,

    Read More

    Question

    I have ordered product ab23457 (mouse monoclonal to mannan binding lectin), and would like to label it. Unfortunately, the buffer it is supplied in contains 15mM azide, which is inhibitory to labeling by succinimydyl esters (according to molecular probes/invitrogen). I would like to use dialysis to remove the excess azide - however, I do not know the precise composition of the buffer the antibody was shipped in (which I need to formulate the buffer to dialyze against). Could you provide me with this information?

    Read More
    Answer

    Thank you for your enquiry. I have been informed by our lab that the antiserum was prepared in 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide. Please do not hesitate to contact me should you require further assistance.

    Read More

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