Anti-Maltose Binding Protein 抗体 (ab9084)

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Key features and details

  • Rabbit polyclonal to Maltose Binding Protein
  • Suitable for: ICC/IF, IP, WB
  • Isotype: IgG

製品の概要

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.00
    Constituent: 50% Glycerol
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/2000.

ターゲット情報

  • 関連性

    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

  • 細胞内局在

    Periplasm

  • 別名

    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

画像

  • Western blot - Anti-Maltose Binding Protein antibody (ab9084)
    Western blot - Anti-Maltose Binding Protein antibody (ab9084)
  • Immunocytochemistry/ Immunofluorescence - Anti-Maltose Binding Protein antibody (ab9084)
    Immunocytochemistry/ Immunofluorescence - Anti-Maltose Binding Protein antibody (ab9084)
    Cell division in a fixed Drosophila melanogaster embryo. The TACC protein is identified in immunofluorescence using the Rabbit polyclonal to MBP which recognises a TACC-MBP fusion.

参考文献 (17)

ab9084 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9084 は 17 報の論文で使用されています。

  • Friedrich M  et al. CREB1 is affected by the microRNAs miR-22-3p, miR-26a-5p, miR-27a-3p, and miR-221-3p and correlates with adverse clinicopathological features in renal cell carcinoma. Sci Rep 10:6499 (2020). PubMed: 32300145
  • Robeson AC  et al. Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators. EMBO J 37:N/A (2018). PubMed: 29875129
  • Wang B  et al. Tomato Yellow Leaf Curl Virus V2 Interacts with Host Histone Deacetylase 6 To Suppress Methylation-Mediated Transcriptional Gene Silencing in Plants. J Virol 92:N/A (2018). PubMed: 29950418
  • Lu Z  et al. Deficiency of PKD2L1 (TRPP3) Exacerbates Pathological Cardiac Hypertrophy by Augmenting NCX1-Mediated Mitochondrial Calcium Overload. Cell Rep 24:1639-1652 (2018). PubMed: 30089272
  • Medina-Puche L  et al. ß-carbonic anhydrases play a role in salicylic acid perception in Arabidopsis. PLoS One 12:e0181820 (2017). PubMed: 28753666
View all Publications for this product

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Western blot abreview for Anti-Maltose Binding Protein antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Bacteria Recombinant protein (BL21-recombinant protein expression)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% SDS)
Loading amount
10 µg
Specification
BL21-recombinant protein expression
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More

Jun Soung Kwak

Verified customer

投稿 Jan 09 2020

Western blot abreview for Anti-Maltose Binding Protein antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
0.5 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Apr 27 2010

Western blot abreview for Anti-Maltose Binding Protein antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Escherichia coli Cell lysate - whole cell (Mach1 (E.coil strain,Invitrogen))
Loading amount
0.05 µg
Specification
Mach1 (E.coil strain,Invitrogen)
Treatment
1mg/ml Lysozyme
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Dec 24 2008

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